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Effect of process parameters and media on the production of ethanol by Scheffersomyces stipitis in shake flask fermentation

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Abstract

The medium composition and fermentation parameters play a critical role in the fermentation process. Therefore, in the present study, the effects of fermentation parameters (initial glucose concentration (IGC), inoculation ratio (IR), initial pH value (IPV), stirring rate (SR), and temperature (T)) and medium composition (yeast extract (YE) and peptone (PEP)) on bioethanol production from glucose by using Scheffersomyces stipitis in shake flask fermentation were studied, respectively. According to results, the highest ethanol yield (YP/S) was achieved to be 47.63% when IGC, IR, IPV, SR, T, and nitrogen concentrations were 15 g/L, 5% (v/v), 5.5, 150 rpm, 30 °C, 5 g/L YE, and 20 g/L PEP, respectively. Nevertheless, the best fermentation parameters were 15 g/L IGC, 5% (v/v) IR, 5.5 IPV, 150 rpm SR, 30 °C T, no YE, and 5 g/L of PEP, respectively. At these conditions, kinetic results including bioethanol production, YP/S, ethanol yield per biomass, maximum production rate, sugar utilization yield, and theoretical YP/S were 8.29 g/L, 47.26%, 5.08 g ethanol/g biomass, 0.41 g/L/h, 95.86%, and 92.49%, respectively. In conclusion, these results indicated that although the concentrations of ingredients (YE and PEP) used in the fermentation medium were considerably decreased from 10 and 20 g/L to 0 and 5 g/L, the kinetic results of the fermentation were preserved compared to the initial fermentation studies. Nevertheless, further improvement of the determined fermentation parameters could be performed in the bioreactor.

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Funding

This work was supported by the Akdeniz University Research Foundation [Grant Number #FBA-2023-6252].

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Authors and Affiliations

  1. Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey

    Mustafa Germec, Ali Ozcan & Irfan Turhan

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  1. Mustafa Germec
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  2. Ali Ozcan
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  3. Irfan Turhan
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Contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Mustafa Germec, Ali Ozcan, and Irfan Turhan. The first draft of the manuscript was written by Mustafa Germec and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Irfan Turhan.

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Germec, M., Ozcan, A. & Turhan, I. Effect of process parameters and media on the production of ethanol by Scheffersomyces stipitis in shake flask fermentation. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-03746-4

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  • DOI: https://doi.org/10.1007/s13399-023-03746-4

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