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Isolation, chemical characterization and in vitro bioactive potential of polysaccharides from seaweed Portieria hornemannii

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Abstract

The goal of this work is to isolate biopolymers from P. hornemannii and assess their chemical composition, structural characteristics and possible antibacterial, antioxidant and anti-diabetic activities. Seaweed P. hornemannii was used to extract the polysaccharides, which were determined to be 34% and contained 1.50% nitrogen, 20.55% carbon and 3.23% of hydrogen. The chemical components such as total carbohydrate, moisture and ash were estimated in polysaccharide from seaweed P. hornemannii and the results were found to be 71, 8 and 3%, respectively. The active functional groups alkyl halide (C–Br), alkyl halide (C-F), alkane (-C-H), alkene (= C-H) and alcohol (O–H) were recorded at the peak between 455.20 and 3429.43 cm−1 by IR spectra and –OH alcohol monomeric, alkyne methine and acetal protons were observed in proto NMR, respectively. In SEM analysis cross linkage, rough surface with porosity and voids surface morphology has been noted. The antibiotic potential of polysaccharides was studied against clinical pathogens and showed maximum activity of 21 and 18 mm zone of inhibition against Vibrio cholerae and Staphylococcus aureus strains at 600 µg/ml concentrations. In antioxidant scavenging screening, the polysaccharide has shown a promising scavenging effect against total antioxidant, DPPH, ferrous chelating and hydroxyl radicals in vitro assays in higher concentrations. The potential antidiabetic effects of polysaccharides were revealed in 79% and 61% α-amylase and β-glucosidase enzymes inhibition at 1000 µg/ml. Seaweed polysaccharide can be used in pharmaceutical industry also as novel multifunctional drug.

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Acknowledgements

The corresponding author M.M. is thankful to the Sathyabama Institute of Science and Technology, Chennai, for providing the Seed Money and lab facilities for doing research work and M.E. and P.A. are thankful to CAS in Marine Biology, Annamalai University, for the providing the lab facilities for doing research work.

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  1. Dr. Meivelu Moovendhan and M. Kavisri contributed equally in this work.

Authors and Affiliations

  1. PG and Research Department of Zoology, Kamaraj College, Thoothukudi, 628003, Tamil Nadu, India

    Manickam Elangovan

  2. CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, 608 502, Tamil Nadu, India

    Perumal Anantharaman

  3. Department of Civil Engineering, School of Building and Environment, Sathyabama Institute of Science and Technology, Chennai, 600119, Tamil Nadu, India

    M. Kavisri

  4. Centre for Ocean Research, Col. Dr. Jeppiaar Research Park, Sathyabama Institute of Science and Technology, Chennai, 600 119, Tamil Nadu, India

    Meivelu Moovendhan

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Meivelu Moovendhan: conceptualization, supervision, data curation, review and editing, funding acquisition, writing—original draft. M. Elangovan: methodology, investigation, resources and formal analysis. P. Anantharaman, M. Kavisri: conceptualization, supervision, review and editing.

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Manickam Elangovan, Perumal Anantharaman, M. Kavisri et al. Isolation, chemical characterization and in vitro bioactive potential of polysaccharides from seaweed Portieria hornemannii. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03276-5

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