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Efficiency of β-glucan production by Sparassis crispa depends on mycelium shape

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Abstract

Sparassis crispa contains various bioactive substances, such as β-glucan, which exhibits antitumor activity. In this study, we investigated the effect of the mycelial shape of S. crispa and the combination of flask type and agitation method on β-glucan production. With the combination of the Erlenmeyer flask and shaken culture, the mycelia grew in the shape of pellets, whereas with the combination of the baffled Erlenmeyer flask and stirred culture, the mycelia grew in the shape of filaments. The dried cell weight (DCW) and β-glucan production of the filamentous mycelia were 5.91 g/L and 1.71 g/L, respectively, 1.34-fold and 1.73-fold higher, respectively, than that of the pelleted mycelia (4.42 g/L and 0.99 g/L, respectively). The production was further increased using the homogenization process; the DCW was 1.03-fold (7.23 g/L) higher and β-glucan production 1.34-fold (3.50 g/L) higher, respectively, than that without the treatment (7.01 g/L and 2.61 g/L, respectively). In the filamentous mycelia, β-glucan production increased with suppressed ethanol production, and a negative correlation was observed between β-glucan production and ethanol production. In the cultivation of S. crispa mycelia, filamentous mycelia have been suggested to be more suitable for β-glucan production than pelleted mycelia.

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Funding

This study was financed in part by the by the Grant-in-Aid for Young Scientists (A) (Grant No. 17H04717) and the Grant-in-Aid for Scientific Research (A) (Grant No. 20H00664) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Department of Biological Science and Technology, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima, 770-8506, Japan

    Ryosuke Okumura

  2. Department of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima, 770-8513, Japan

    Yoshitoshi Nakamura & Chikako Asada

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  1. Ryosuke Okumura
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  2. Yoshitoshi Nakamura
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Ryosuke Okumura: conceptualization, methodology, writing-original. Yoshitoshi Nakamura: investigation, data curation and analysis, writing-review and editing. Chikako Asada: methodology, validation, funding acquisition, supervision.

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Correspondence to Chikako Asada.

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Okumura, R., Nakamura, Y. & Asada, C. Efficiency of β-glucan production by Sparassis crispa depends on mycelium shape. Biomass Conv. Bioref. 14, 1939–1947 (2024). https://doi.org/10.1007/s13399-022-02555-5

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