Abstract
A quick fed-batch saccharification (QFBs) strategy of pretreated wheat straw at high solid loadings (HSL) was developed to achieve high sugar concentrations and increase lignocellulosic ethanol productivities in the fermentation stage. Pretreatment time, number of feedings, and the time between them were evaluated using a Box-Behnken design. A surface response model was employed to maximize sugars released at 20% (w/w) HSL. Total sugar concentrations after 60 h with 20% (w/w), 25% (w/w), and 30% (w/w) HSL were 128.5 g/l, 142.4 g/l, and 163.5 g/l, respectively. These were 14.6–42% higher than their batch saccharification counterparts. The QFBs was implemented as part of sequential (SHF) and simultaneous saccharification-fermentation (SSF) strategies as well as the presaccharification stage of a semi-simultaneous saccharification-fermentation (SSSF) strategy. In the latter strategy, the highest ethanol concentration achieved was 31.7 g/l. The ethanol volumetric productivity was 2.2- and 3-fold their SHF and SSF counterparts.
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Acknowledgements
The authors also acknowledge Dr. J. A. Perez-Pimienta and Dr. F. Rodriguez-Palomera for their assistance in the pretreatment of WS using the CR at the Bioenergy Futures Laboratory.
Funding
Financial support was received from the Energy Sustainability Fund 2014 (CONACYT-SENER, Mexico) Grants 245750 and 249564 (Mexican Bioenergy Innovation Centre, Bio-alcohols Cluster). JG received financial support from CONACYT in the form of Ph.D. scholarships (455101 and 291250).
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All authors contributed to the study’s conception and design. The conceptualization of the work was established by Jorge Gonzalez, Arturo Sanchez, and Lorena Amaya. Material preparation, data collection, and analysis were performed by Jorge Gonzalez and Alexander Valle. The first draft of the manuscript was written by all authors who commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Gonzalez-Rios, J.A., Valle-Pérez, A.U., Amaya-Delgado, L. et al. A quick fed-batch saccharification strategy of wheat straw at high solid loadings improving lignocellulosic ethanol productivity. Biomass Conv. Bioref. 13, 4991–5003 (2023). https://doi.org/10.1007/s13399-021-01580-0
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DOI: https://doi.org/10.1007/s13399-021-01580-0