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Antibacterial Activity of Lactobacillus plantarum CRL 759 Against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

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Abstract

This study investigates the antibacterial properties of potential probiotic Lactobacillus plantarum CRL 759 against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) isolated from samples of human diabetic foot, by different methods. The results demonstrated that L. plantarum CRL 759 cell-free supernatant (SLp759) inhibited both P. aeruginosa and MRSA growth. In addition, SLp759 inhibited the adhesion of pathogenic microbes. The characterization of SLp759 showed that after neutralization of acidic SLp759, no activity against pathogens strains was observed. Moreover, treatment with proteolytic enzymes did not modify antibacterial activity, indicating that no bacteriocin was present in the supernatant. In addition, results obtained by HPLC analysis indicated that the inhibitory effect was the result of production of two organic acids: lactic and acetic. From the results reported here, L. plantarum CRL 759 has potential applications as a probiotic by preventing or breaking multidrug-resistant microorganism carriage.

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Acknowledgements

The authors thank Bioq. Melisa Turpo (Hospital de Clínicas Nicolás Avellaneda, Tucumán, Argentina.) and Dr. Clara Silva (Cátedra de Bacteriología, Facultad de Bioquímica, Química y Farmacia- UNT) for providing the pathogenic strains. This work was supported by grant from Agencia Nacional de Promoción Científica y Tecnológica (PICT 0786) (PIP 384).

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  1. Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, Tucumán, Argentina

    Barbara I. Layus, Carla L. Gerez & Ana V. Rodriguez

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  1. Barbara I. Layus
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  2. Carla L. Gerez
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  3. Ana V. Rodriguez
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Correspondence to Ana V. Rodriguez.

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Layus, B.I., Gerez, C.L. & Rodriguez, A.V. Antibacterial Activity of Lactobacillus plantarum CRL 759 Against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa. Arab J Sci Eng 45, 4503–4510 (2020). https://doi.org/10.1007/s13369-020-04491-w

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  • DOI: https://doi.org/10.1007/s13369-020-04491-w

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