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Photopolymerized PVA-g-GMA Hydrogels for Biomedical Applications: Factors Affecting Hydrogel Formation and Bioevaluation Tests

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Abstract

A series of PVA-g-GMA crosslinked hydrogel based on poly(vinyl alcohol) (PVA) functionalized with vinyl groups of glycidyl methacrylate (GMA) has been synthesized. The trans-esterification reaction occurred by introducing methacryloyl groups into PVA chains, while the glycidol was produced as by-product. The water-soluble PVA-g-GMA macromonomers were photocrosslinked using Irgacure 2959 (\(\hbox {I}_{2959})\) as UV photoinitiator at 365 nm. Factors affecting the hydrogel formation, e.g., PVA-g-GMA polymer concentration, \(\hbox {I}_{2959}\) initiator concentration, and irradiation time, were discussed as a function with the swelling degree of formed hydrogels. Also, in vitro bioevaluation tests have been assessed. It was realized that the swelling degree of PVA-g-GMA hydrogels was reduced significantly with increasing the GMA concentration, prolonging the irradiation time and initiator concentration to certain extent. Notably, high GMA content in PVA-g-GMA hydrogels exhibited low protein adsorption, high thermal stability, and very slow hydrolytic degradation, due to the high crosslinking density and tighter structure of the formed hydrogels. However, hydrogels do not show antimicrobial activity against E. coli and klebsiella pneumonia growth even with loaded ampicillin, while unexpected significant resistance was observed against Staphylococcus aureus owing to the loaded ampicillin in hydrogels. Surprisingly, PVA-g-GMA with high GMA contents showed cytotoxic effect against cells using MTT assay, compared to the safe moderated GMA content hydrogels.

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Authors and Affiliations

  1. Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab, Alexandria, 21934, Egypt

    Elbadawy A. Kamoun, Ahmed M. Omer & Heba M. Ahmed

  2. Medical Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg Al-Arab, Alexandria, 21934, Egypt

    Marwa M. Abu-Serie

  3. Chemistry Department, Faculty of Science, Alexandria University, P.O. Box 426, Ibrahimia, Alexandria, 21321, Egypt

    Sherine N. Khattab & Ali A. Elbardan

Authors
  1. Elbadawy A. Kamoun
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  2. Ahmed M. Omer
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  3. Marwa M. Abu-Serie
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  4. Sherine N. Khattab
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  5. Heba M. Ahmed
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  6. Ali A. Elbardan
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Correspondence to Elbadawy A. Kamoun.

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Kamoun, E.A., Omer, A.M., Abu-Serie, M.M. et al. Photopolymerized PVA-g-GMA Hydrogels for Biomedical Applications: Factors Affecting Hydrogel Formation and Bioevaluation Tests. Arab J Sci Eng 43, 3565–3575 (2018). https://doi.org/10.1007/s13369-017-3054-5

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  • DOI: https://doi.org/10.1007/s13369-017-3054-5

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