Abstract
In the current study, a 58-year-old male patient presented with recurrent glioblastoma multiforme (GBM). The patient underwent surgical resection, 4 months earlier, followed by radiotherapy and chemotherapy. During the second surgical intervention, tumour tissue and whole blood were sampled and analysed for human cytomegalovirus (HCMV) DNA, immediate early (IE) mRNA and pp65 mRNA. HCMV DNA was detected only in the recurrent tumour tissue but not in the whole blood. Neither IE mRNA nor pp65 mRNA was expressed. Our result suggests HCMV latency in the brain tumour with detectable level of viral DNA. More data are needed to understand the HCMV infection chronology in the brain tumours but our data could be important for further studies of HCMV antigens on the tumour surface and anti-GBM therapy.
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Acknowledgements
We thank Teodora Tasseva from Institute of Plant Physiology and Genetics – Bulgarian Academy of Sciences, Slavena Atemin and Zornitsa Pavlova from Genetic Medico-Diagnostic Laboratory “Genica”, Sofia, Bulgaria.
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This work was supported in part by Medical University – Sofia, grants D-118/2018 and D-127/2019.
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Nikolova, E., Dimova, P., Minkin, K. et al. Human cytomegalovirus DNA detection in a recurrent glioblastoma multiforme tumour, but not in whole blood: a case report and discussion about the HCMV latency and therapy perspectives. J. Neurovirol. 26, 984–987 (2020). https://doi.org/10.1007/s13365-020-00901-9
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DOI: https://doi.org/10.1007/s13365-020-00901-9