Abstract
Transmission efficiencies of capsicum chlorosis virus (CaCV) were investigated for four thrips species (Thysanoptera: Thripidae), Frankliniella occidentalis (Pergande), Frankliniella intonsa (Trybom), Thrips palmi (Karny), and Thrips tabaci Lindeman infesting green pepper in Japan. The first-instar larvae of the thrips were allowed to feed on CaCV-infected green pepper leaves for 24 h. The transmission of CaCV by thrips was examined using a petunia leaf disk assay. CaCV was transmitted by four male (8.70%) and four female (3.88%) T. palmi, but the virus was not transmitted by adults of any other species. In a second transmission assay, 10 adult T. palmi that had fed on infected leaves as larvae were released to healthy green pepper seedlings. After 21 days, the seedlings were examined and 50% of them showed symptoms of CaCV infection. RT-PCR confirmed that they were infected with the virus. The viruliferous rate of adult T. palmi was 60%, while CaCV was not detected in adults of the other three species. These results suggest that T. palmi is capable of being a new vector of CaCV.
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References
Abudurexiti A, Adkins S, Alioto D et al (2019) Taxonomy of the order Bunyavirales: update 2019. Arch Virol 164:1949–1965. https://doi.org/10.1007/s00705-019-04253-6
Chen CC, Huang CH, Chen TC, Yeh SD, Cheng YH, Hsu HT, Chang CA (2007a) First report of Capsicum chlorosis virus causing yellow stripes on calla lilies in Taiwan. Plant Dis 91:1201. https://doi.org/10.1094/PDIS-91-9-1201C
Chen K, Xu Z, Yan L, Wang G (2007b) Characterization of a new strain of Capsicum chlorosis virus from peanut (Arachis hypogaea L.) in China. J Phytopathol 155:178–181. https://doi.org/10.1111/j.1439-0434.2007.01217.x
German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu Rev Phytopathol 30:315–348. https://doi.org/10.1146/annurev.py.30.090192.001531
Huang CH, Zheng YX, Cheng YH, Lee WS, Jan FJ (2010) First report of Capsicum chlorosis virus infecting tomato in Taiwan. Plant Dis 94:1263. https://doi.org/10.1094/PDIS-04-10-0275
Inoue T, Sakurai T, Murai T, Maeda T (2004) Specificity of accumulation and transmission of tomato spotted wilt virus (TSWV) in two genera, Frankliniella and Thrips (Thysanoptera: Thripidae). Bull Entomol Res 94:501–507. https://doi.org/10.1079/BER2004326
Kimura K, Usugi T, Hoshi H, Kato A, Ono T, Koyano S, Kagiwada S, Nishio T, Tsuda S (2016) Surveys of viruliferous alate aphid of Plum pox virus in Prunus mume orchards in Japan. Plant Dis 100:40–48. https://doi.org/10.1094/PDIS-05-15-0540-RE
Knierim D, Blawid R, Maiss E (2006) The complete nucleotide sequence of a capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch Virol 151:1761–1782. https://doi.org/10.1007/s00705-006-0749-4
Krishnareddy M, Rani RU, Kumar KA, Reddy KM, Pappu HR (2008) Capsicum chlorosis virus (genus Tospovirus) infecting chili pepper (Capsicum annuum) in India. Plant Dis 92:1469. https://doi.org/10.1094/PDIS-92-10-1469B
Kunkalikar S, Poojari S, Rajagopalan P, Zehr UB, Naidu RA, Kankanallu RS (2007) First report of Capsicum chlorosis virus in tomato in India. Plant Health Prog. https://doi.org/10.1094/PHP-2007-1204-01-BR
McMichael LA, Persley DM, Thomas JE (2000) The first record of a serogroup IV tospovirus in Australia. Australas Plant Pathol 29:149. https://doi.org/10.1071/AP00023
McMichael LA, Persley DM, Thomas JE (2002) A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia. Australas Plant Pathol 31:231–239. https://doi.org/10.1071/AP02016
Melzer MJ, Shimabukuro J, Long MH, Nelson SC, Alvarez AM, Borth WB, Hu JS (2014) First report of Capsicum chlorosis virus infecting waxflower (Hoya calycina Schlecter) in the United States. Plant Dis 98:571. https://doi.org/10.1094/PDIS-06-13-0588-PDN
Okuda M (2016) Tospoviruses occurring in and outside Japan. Jpn J Phytopathol 82:169–184. https://doi.org/10.3186/jjphytopath.82.169(in Japanese with English summary)
Okuda S, Okuda M, Matsuura S, Okazaki S, Iwai H (2013) Competence of Frankliniella occidentalis and Frankliniella intonsa strains as vectors for Chrysanthemum stem necrosis virus. Eur J Plant Pathol 136:355–362. https://doi.org/10.1007/s10658-013-0169-8
Persley DM, Thomas JE, Sharman M (2006) Tospoviruses—an Australian perspective. Australas Plant Pathol 35:161–180. https://doi.org/10.1071/AP06015
Premachandra WTSD, Borgemeister C, Maiss E, Knierim D, Poehling HM (2005) Ceratothripoides claratris, a new vector of a Capsicum chlorosis virus isolate infecting tomato in Thailand. Phytopathology 95:659–663. https://doi.org/10.1094/PHYTO-95-0659
Rotenberg D, Jacobson AL, Schneweis DJ, Whitfield AE (2015) Thrips transmission of tospoviruses. Curr Opin Virol 15:80–89. https://doi.org/10.1016/j.coviro.2015.08.003
Sakurai T, Murai T, Maeda T, Tsumuki H (1998) Sexual differences in transmission and accumulation of tomato spotted wilt virus in its insect vector Frankliniella occidentalis (Thysanoptera: Thripidae). Appl Entomol Zool 33:583–588. https://doi.org/10.1303/aez.33.583
Sakurai T, Inoue T, Tsuda S (2004) Distinct efficiencies of Impatiens necrotic spot virus transmission by five thrips vector species (Thysanoptera: Thripidae) of tospoviruses in Japan. Appl Entomol Zool 39:71–78. https://doi.org/10.1303/aez.2004.71
Tyagi K, Kumar V, Singha D, Chandra K, Laskar BA, Kundu S, Chakraborty R, Chatterjee S (2017) DNA Barcoding studies on Thrips in India: cryptic species and species complexes. Sci Rep 7:4898. https://doi.org/10.1038/s41598-017-05112-7
Ullman DE, German TL, Sherwood JL, Westcot DM, Cantone FA (1993) Tospovirus replication in insect vector cells: immunocytochemical evidence that the nonstructural protein encoded by the S RNA of tomato spotted wilt tospovirus is present in thrips vector cells. Phytopathology 83:456–463. https://doi.org/10.1094/Phyto-83-456
Ullman DE, Westcot DM, Chenault KD, Sherwood JL, German TL, Bandla MD, Cantone FA, Duer HL (1995) Compartmentalization, intracellular transport, and autophagy of tomato spotted wilt tospovirus proteins in infected thrips cells. Phytopathology 85:644–654. https://doi.org/10.1094/Phyto-83-456
van de Wetering F, van der Hoek M, Goldbach R, Peters D (1999) Differences in tomato spotted wilt virus vector competency between males and females of Frankliniella occidentalis. Entomol Exp Appl 93:105–112. https://doi.org/10.1046/j.1570-7458.1999.00567.x
Wijkamp I, Peters D (1993) Determination of the median latent period of two tospoviruses in Frankliniella occidentalis, using a novel leaf disk assay. Phytopathology 83:986–991. https://doi.org/10.1094/Phyto-83-986
Wijkamp I, van Lent J, Kormelink R, Goldbach R, Peters D (1993) Multiplication of tomato spotted wilt virus in its insect vector, Frankliniella occidentalis. J Gen Virol 74:341–349. https://doi.org/10.1099/0022-1317-74-3-341
Wijkamp I, Almarza N, Goldbach R, Peters D (1995) Distinct levels of specificity in thrips transmission of tospoviruses. Phytopathology 85:1069–1074. https://doi.org/10.1094/Phyto-85-1069
Zheng YX, Chen CC, Jan FJ (2011) Complete nucleotide sequence of capsicum chlorosis virus isolated from Phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses. Arch Virol 156:421–432. https://doi.org/10.1007/s00705-010-0874-y
Acknowledgements
This work was supported by Cabinet Office, Government of Japan, Cross-ministerial Strategic Innovation Promotion Program (SIP), “Technologies for creating next-generation agriculture, forestry and fishers” (funding agency: Bio-oriented Technology Research Advancement Institution, NARO). We would like to thank Dr. Shuichi Yamasaki (Oita Prefectural Agriculture, Forestry and Fisheries Research Center) for providing the CaCV-OITA isolate, and Dr. Takeshi Ohya (Kanagawa Agricultural Technology Center) and Dr. Kazuhiro Komi (Kochi Agricultural Research Center) for providing cultures of thrips populations.
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Chiaki, Y., Kubota, K., Tomitaka, Y. et al. Transmission of capsicum chlorosis virus by Thrips palmi (Thysanoptera: Thripidae). Appl Entomol Zool 55, 31–35 (2020). https://doi.org/10.1007/s13355-019-00649-7
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DOI: https://doi.org/10.1007/s13355-019-00649-7