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Bioefficacy of entomopathogenic Aspergillus strains against the melon fly, Bactrocera cucurbitae (Diptera: Tephritidae)

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Abstract

Three Aspergillus strains were isolated from naturally diseased adults of the melon fly Bactrocera cucurbitae Coquillett (Diptera: Tephritidae) collected from Hainan Province, China. Then laboratory bioassays were carried with A. flavus (BC-22 and BC-D-1) and A. tamarii strains (BC-212) against B. cucurbitae. The results indicated that conidial suspensions (1 × 108 conidia/ml) of the three strains exhibited high toxicity against the adults of B. cucurbitae and low insecticidal activity to the eggs, larvae and pupae. Furthermore, the virulence of three strains (BC-22, BC-D-1 and BC-212) for the adults was compared; the LT50 values were 6.05, 5.81 and 4.91 days with 1 × 108 conidia/ml, respectively. After 8 days after inoculation, the LC50 values were 12.00 × 105, 1.15 × 105 and 0.28 × 105 conidia/ml, respectively. Undiluted culture supernatant of the three strains had less bioefficacy to B. cucurbita compared to use of the conidia suspension. The three strains, especially BC-212, showed a faster colony growth, higher germination rate and sporulation. These results indicated that the A. tamarii BC-212 strain was the better one to consider for the control of melon flies. In conclusion, Aspergillus species could be candidates for fly control as pest control agents. To our knowledge, this is the first report on A. flavus and A. tamarii strains pathogenic to B. cucurbitae.

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References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18(2):265–267

    Article  CAS  Google Scholar 

  • Amala U, Jiji T, Naseema A (2013) Laboratory evaluation of local isolate of entomopathogenic fungus, Paecilomyces lilacinus Thom Samson (ITCC 6064) against adults of melon fruit fly, Bactrocera cucurbitae Coquillett (Diptera: Tephritidae). J Trop Agri 51:132–134

    Google Scholar 

  • Bawin T, Seye F, Boukraa S, Zimmer JY, Delvigne F, Ndiaye M, Francis F (2013) Application of three entomopathogenic fungi for aphid control. 65th International Symposium on Crop Protection-May 2013-Ghent University. http://www.hdl.handle.net/2268/151303. Accessed 21 May 2013

  • Bhan S, Shrankhla Mohan L, Srivastava CN (2013) Larvicidal toxicity of temephos and entomopathogenic fungus, Aspergillus flavus and their synergistic activity against malaria vector, Anopheles stephensi. J Entomol Zool Stu 1(6):55–60

    Google Scholar 

  • Castillo MA, Moya P, Hernández E, Primo-Yúfera E (2000) Susceptibility of Ceratitis capitata Wiedemann (Diptera: Tephritidae) to entomopathogenic fungi and their extracts. Biol Control 19(3):274–282

    Article  Google Scholar 

  • Dhillon MK, Singh R, Naresh JS, Sharma HC (2005) The melon fruit fly, Bactrocera cucurbitae: a review of its biology and management. J Insect Sci 5:40

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dimbi S, Maniania NK, Ekesi S (2009) Effect of Metarhizium anisopliae inoculation on the mating behavior of three species of African Tephritid fruit flies, Ceratitis capitata, Ceratitis cosyra and Ceratitis fasciventris. Biol Control 50(2):111–116

    Article  Google Scholar 

  • Fabre FB, Ryckewaert PB, Duyck PF (2003) Comparison of the efficacy of different food attractants and their concentration for melon fly (Diptera: Tephritidae). J Econo Entomol 96(1):231–238

    Article  CAS  Google Scholar 

  • Fernández-Marín H, Zimmerman JK, Wcislo WT (2004) Ecological traits and evolutionary sequence of nest establishment in fungus growing ants (Hymenoptera Formicidae, Attini). Biol J Linn Soc 81:39–48

    Article  Google Scholar 

  • Hughes WOH, Eilenberg J, Boomsma JJ (2002) Trade-offs in group living: transmission and disease resistance in leaf-cutting ants. Proc R Soc Lond B 269:1811–1819

    Article  Google Scholar 

  • Jiang CM, Ai HM, Zhao SX (2005) The risk analysis of fly (Bactrocera cucurbitae). Entomol J East China 14(3):256–259 (in Chinese with English summary)

    Google Scholar 

  • Kong LB, Lin W, Li ZH, Wan FH, Wang ZL, Huang GS (2008) A predication of potential geographic distribution of melon fruit fly based on CLIMEX and DIVA-GIS. Acta Phytophy Sin 2:14–154

    Google Scholar 

  • Li GP, Liu F, Chang JM, Zhao YL, He YB, Zhan RL (2012) Trapping effect of different food baits attractant on Bactrocera (Zeugodacus) cucurbitae (Coquillett)in Bitter Melon Park. China Vegetables 2:79–82 (in Chinese with English summary)

    Google Scholar 

  • Li ZH, Jiang F, Ma XL, Fang Y, Sun ZZ, Qin YJ, Wang QL (2013) Review on prevention and control techniques of Tephritidae invasion. Plant Quar 2:1–10 (in Chinese with English summary)

    Google Scholar 

  • Ma XY, Wang M, Yang Y, Zu D, Zhang CN (2013) Biological characteristics and toxicity determination to Bactrocera cucurbitae. Guangdong Agri Sci 40(6):77–79 (in Chinese with English summary)

    CAS  Google Scholar 

  • Pinero JC, Jaccome I, Vargas R, Ronald JP (2006) Response of female melon fly, Bactrocera cucurbitae, to host-associated visual and olfactory stimuli. Entomol Exp Appl 121:261–269

    Article  Google Scholar 

  • Poulsen M, Hughes WOH, Boomsma JJ (2006) Differential resistance and the importance of antibiotic production in Acromyrmex echinatior leaf-cutting ant castes towards the entomopathogenic fungus Aspergillus nomius. Insect Soc 53:1–7

    Article  Google Scholar 

  • Qi ZT (1997) Flora fungorum sinicorum. Aspergillus et teleomorphi cognati, vol 5. Science Press, Beijing (in Chinese)

    Google Scholar 

  • Robert K, Mensah Leah A (2012) Microbial control of cotton pests. Part I: Use of the naturally occurring entomopathogenic fungus Aspergillus sp. (BC 639) in the management of Creontiades dilutus (Stal) (Hemiptera: Miridae) and beneficial insects on transgenic cotton crops. Biocontrol Sci Techn 22(5):567–582

    Article  Google Scholar 

  • Sapkota R, Dahal KC, Thapa RB (2010) Damage assessment and management of cucurbit fruit flies in spring-summer squash. J Entomo Nematol 2(1):7–12

    Google Scholar 

  • Seye F, Faye O, Ndiaye M, Njie E, Afoutou JM (2009) Pathogenicity of the Fungus, Aspergillus clavatus, isolated from the locust, Oedaleus senegalensis, against larvae of the mosquitoes Aedes aegypti, Anopheles gambiae and Culex quinquefasciatus. J Insect Sci 9:1–7

    Article  PubMed  Google Scholar 

  • Seye F, Bawin T, Boukraa S, Zimmer JY, Ndiaye M, Delvigne F, Francis F (2014) Effect of entomopathogenic Aspergillus strains against the pea aphid, Acyrthosiphon pisum (Hemiptera: Aphididae). Appl Etomol Zool 49(3):453–458

    Article  Google Scholar 

  • Sookar P, Bhagwant S, Ouna EA (2008) Isolation of entomopathogenic fungi from the soil and their pathogenicity to two fruit fly species (Diptera: Tephritidae). J Appl Entomol 132(9):778–788

    Article  Google Scholar 

  • Verghese A, Sreedevi K, Nagaraju DK (2006) Pre and post harvest IPM for the management of mango fruit fly Bactrocera dorsalis (Hendel). In: Proc. of Seventh Int. Sym. on Fruit flies of Economic Importance, 10–15 September, Salvador, Brazil, pp 179–182

  • Yuan SY, Kong Q, Tian XJ, Guo YL, Chen B, Li X (2013) Study on Laboratory toxicity of Beauveria bassiana strain to Bactrocera cucurbitae Coquillett. Northern Horticulture 7:137–140 (in Chinese with English summary)

    Google Scholar 

Download references

Acknowledgments

This work was supported by the Project of the Science and Technology Industry—Invasive Insects Comprehensive Prevention and Control Technology Research and Demonstration promotion (201103026-1) and Midwest University Project (MWECSP-RT08, ZXBJH-XK004 and ZXBJH-XK005). We thank Cheng Bai, the Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, for critical review and modification of the text.

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Authors and Affiliations

  1. The Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, 570228, Hainan, China

    Ye Yang, Yu Zhang, Meng Wang, Shan-shan Li, Xiao-yan Ma & Zhan-hua Xu

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  1. Ye Yang
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  2. Yu Zhang
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  3. Meng Wang
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  4. Shan-shan Li
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  5. Xiao-yan Ma
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Correspondence to Ye Yang.

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Yang, Y., Zhang, Y., Wang, M. et al. Bioefficacy of entomopathogenic Aspergillus strains against the melon fly, Bactrocera cucurbitae (Diptera: Tephritidae). Appl Entomol Zool 50, 443–449 (2015). https://doi.org/10.1007/s13355-015-0351-x

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