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Cell-culture derived fowl adenovirus serotype 4 inactivated vaccine provides complete protection for virus infection on SPF chickens

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Abstract

Inclusion body hepatitis and hepatitis–hydropericardium syndrome caused by high-pathogenic fowl adenovirus serotype 4 has recently plagued Chinese poultry industry and caused huge economic losses since 2013. So far, there is no commercial vaccine available to control this disease. In this study, we reported the development of both embryo-adapted and cell-culture derived inactivated FAdV-4 vaccines and evaluated their efficacies in chicken. Compared to embryo-adapted vaccine, cell-culture derived vaccine induced significantly earlier and higher serological response measured by AGP and ELISA. After virus challenge, chicken immunized with cell-culture derived vaccine did not showed any gross and histopathological lesions, whereas inclusion body hepatitis was observed in the liver of chicken vaccinated with embryo-adapted vaccine. No mortality was observed in both the vaccinated groups. The above results suggested that cell-culture derived FAdV-4 inactivated vaccine could be a better vaccine candidate than embryo-adapted vaccine to control FADV-4 infections in China.

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Acknowledgement

This work was supported by Grant from Luoyang Heluo Talent Plan (Dr. Kegong Tian).

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Author notes

  1. Dongying Du, Pantao Zhang, and Xiangdong Li have contributed equally to this study.

Authors and Affiliations

  1. National Research Center for Veterinary Medicine, Cuiwei Road, High-Tech District, Luoyang, 471003, China

    Dongying Du, Pantao Zhang, Xiangdong Li, Hui Tian, Yi Cheng, Dongbei Sheng, Xueying Han, Yihong Shan, Xuefeng Li, Yue Yuan, Haiyang Zhang, Jingjing Xue, Wujie Liu & Kegong Tian

  2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China

    Kegong Tian

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  1. Dongying Du
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Correspondence to Wujie Liu or Kegong Tian.

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Du, D., Zhang, P., Li, X. et al. Cell-culture derived fowl adenovirus serotype 4 inactivated vaccine provides complete protection for virus infection on SPF chickens. VirusDis. 28, 182–188 (2017). https://doi.org/10.1007/s13337-017-0372-x

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  • DOI: https://doi.org/10.1007/s13337-017-0372-x

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