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Targeted nanobubbles in low-frequency ultrasound-mediated gene transfection and growth inhibition of hepatocellular carcinoma cells

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Tumor Biology

Abstract

The use of SonoVue combined with ultrasound exposure increases the transfection efficiency of short interfering RNA (siRNA). The objective of this study was to prepare targeted nanobubbles (TNB) conjugated with NET-1 siRNA and an antibody GPC3 to direct nanobubbles to hepatocellular carcinoma cells. SMMC-7721 human hepatocellular carcinoma cells were treated with six different groups. The transfection efficiency and cellular apoptosis were measured by flow cytometry. The protein and messenger RNA (mRNA) expression were measured by Western blot and quantitative real-time PCR, respectively. The migration and invasion potential of the cells were determined by Transwell analysis. The results show that US-guided siRNA-TNB transfection effectively enhanced gene silencing. In summary, siRNA-TNB may be an effective delivery vector to mediate highly effective RNA interference in tumor treatment.

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Authors and Affiliations

  1. Department of Ultrasound, Harbin Medical University Cancer Hospital, Harbin Medical University Cancer Hospital, Harbin, 150081, Heilongjiang Province, China

    Bolin Wu, Qiang Qiao, Xue Han, Hui Jing & Wen Cheng

  2. School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, China

    Hao Zhang & Hongjian Liang

Authors
  1. Bolin Wu
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  2. Qiang Qiao
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  3. Xue Han
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  4. Hui Jing
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  5. Hao Zhang
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  6. Hongjian Liang
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  7. Wen Cheng
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Corresponding author

Correspondence to Wen Cheng.

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Grant support

This study was financially supported by the National Natural Science Foundation of China (#81371568).

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Wu, B., Qiao, Q., Han, X. et al. Targeted nanobubbles in low-frequency ultrasound-mediated gene transfection and growth inhibition of hepatocellular carcinoma cells. Tumor Biol. 37, 12113–12121 (2016). https://doi.org/10.1007/s13277-016-5082-2

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  • DOI: https://doi.org/10.1007/s13277-016-5082-2

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