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DCN deficiency promotes renal cell carcinoma growth and metastasis through downregulation of P21 and E-cadherin

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Tumor Biology

Abstract

Decorin (DCN), as an important component of the extracellular matrix (ECM), is a small leucine-rich proteoglycan and synthesized by fibroblasts. Although DCN is dysregulated in numerous cancer types, limited data are available on the expression level and important role of DCN proteins in renal cell carcinoma (RCC). In our study, we examined the expression patterns of DCN messenger RNA (mRNA) in RCCs through the Oncomine database and DCN protein in 94 RCC specimens by immunohistochemistry (IHC). The results revealed that DCN expression was decreased in cancerous tissues compared to adjacent noncancerous tissues and was highly correlated to tumor size. Then, via gain-of-function analyses, DCN overexpression could inhibit RCC cell proliferation and metastasis in vitro and vivo. At the mechanism level, we found that an ectopic expression of DCN significantly upregulated P21 and E-cadherin expression. Altogether, these results revealed that DCN is a tumor suppressor in RCC, and it could serve as a potential therapeutic target in patients with RCC.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 81171391 and No. 81372743). We apologize to all the researchers whose relevant contributions were not cited due to space limitations.

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Authors and Affiliations

  1. Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing, 210002, Jiangsu Province, China

    Yongcan Xu, Qiuyuan Xia, Qiu Rao, Shanshan Shi, Qunli Shi, Henghui Ma, Zhenfeng Lu, Hui Chen & Xiaojun Zhou

  2. Department of General Surgery, Huzhou Central Hospital, Huzhou, China

    Yongcan Xu

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  1. Yongcan Xu
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  2. Qiuyuan Xia
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  3. Qiu Rao
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  4. Shanshan Shi
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  9. Xiaojun Zhou
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Correspondence to Xiaojun Zhou.

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The study protocol was approved by the Institutional Review Board of Nanjing University, and all of the participants signed an informed consent form.

The tests on animals were conducted according to Institutional Animal Care and User guidelines and experimental procedures were approved by the Model Animal Research Center of Jingling Hospital.

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Xu, Y., Xia, Q., Rao, Q. et al. DCN deficiency promotes renal cell carcinoma growth and metastasis through downregulation of P21 and E-cadherin. Tumor Biol. 37, 5171–5183 (2016). https://doi.org/10.1007/s13277-015-4160-1

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  • DOI: https://doi.org/10.1007/s13277-015-4160-1

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