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Loss of β-arrestin1 expression predicts unfavorable prognosis for non-small cell lung cancer patients

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Tumor Biology

Abstract

We aimed to study the expression status of β-arrestin1 in non-small cell lung cancer (NSCLC) specimens and its clinicopathologic significance. The correlation between β-arrestin1 and the tumor migration biomarker E-cadherin, as well as smoking index were studied. A total of 152 patients with NSCLC who undergone surgery were enrolled. Altogether, 88 lung squamous cell lung cancer (SCC) specimens and 64 adenocarcinoma (ADC) specimens were tested for immunohistochemistry. Patients’ survival was analyzed by the Kaplan–Meier method. Univariate and multivariate analyses were performed to determine independent prognostic factors. Spearman rank correlation test was used to show data associations. For SCC patients, the expression of β-arrestin1 was either lost (56 of 88, 63.6 %) or low (32 of 88, 36.4 %), which was significantly and negatively associated with E-cadherin expression (P = 0.017). The similar correlation existed between smoking index and β-arrestin1 expression (P = 0.044). For ADC patients, the deletion of β-arrestin1 expression was rare (4 of 64, 6.3 %). Loss of β-arrestin1 expression indicated poorer survival for both SCC (P = 0.026) and ADC patients (P = 0.006). β-arrestin1 expression was detected in the other ADC specimens but showed no significant correlation with survival. In SCC patients, the loss expression of β-arrestin1 was frequently observed, and β-arrestin1 expression was significantly correlated with the smoking index and E-cadherin expression, which all indicated β-arrestin1’s significant clinicopathologic role. However, β-arrestin1 was expressed in most ADC patients, but its clinicopathologic role seemed to be obscure and might need further exploration.

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Acknowledgments

The authors thank doctors Lin and Liu of the Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong University, for their assistance in the processing of tissue sections and the assessment of immunostaining results. This work was supported by Provincial Science and Technology Development Planning of Shandong (2012G0021836), Shandong Provincial Natural Science Foundation of China (ZR2013HZ001), and National Natural Science Foundation of China (81301728).

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None

Research involving human participants and/or animals

Our research was approved by Ethical Committee of Shandong Provincial Hospital affiliated to Shandong University.

Informed consent

The informed written consent for the use of their clinical study was obtained from every investigated patients.

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    Authors and Affiliations

    1. Department of Thoracic Surgery, Shandong Provincial Hospital, Shandong University, 324 Jingwu Road, Jinan, 250021, People’s Republic of China

      Honghai Ma & Jiajun Du

    2. Department of Thoracic Surgery, First Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, People’s Republic of China

      Honghai Ma

    3. Institute of Oncology, Shandong Provincial Hospital, Shandong University, 324 Jingwu Road, Jinan, 250021, People’s Republic of China

      Liguang Wang, Tiehong Zhang, Hongchang Shen & Jiajun Du

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    1. Honghai Ma
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    Correspondence to Jiajun Du.

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    Honghai Ma and Liguang Wang contributed equally to this work.

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    Ma, H., Wang, L., Zhang, T. et al. Loss of β-arrestin1 expression predicts unfavorable prognosis for non-small cell lung cancer patients. Tumor Biol. 37, 1341–1347 (2016). https://doi.org/10.1007/s13277-015-3886-0

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    • DOI: https://doi.org/10.1007/s13277-015-3886-0

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