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LRRC3B is downregulated in non-small-cell lung cancer and inhibits cancer cell proliferation and invasion

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Tumor Biology

Abstract

LRRC3B has emerged as a tumor suppressor in several human cancers. However, its expression pattern and biological roles in human non-small-cell lung cancer (NSCLC) have not been explored. In the present study, we investigated clinical significance of LRRC3B in 101 NSCLC specimens. We found that LRRC3B expression was downregulated in NSCLC tissues compared with normal bronchial epithelium and that its downregulation significantly correlated with tumor–node–metastasis (TNM) stage (p < 0.0001), nodal metastasis (p < 0.0001), and poor patient prognosis (p = 0.0016, log-rank test). We also checked LRRC3B levels in several lung cancer cell lines and found that its expression was downregulated in four of nine lung cancer cell lines compared with normal human bronchial epithelial (NHBE) cell line. We further explored the biological role of LRRC3B. LRRC3B plasmid transfection in H460 and A549 cell lines inhibited proliferation, colony formation ability, and invading ability. Furthermore, we identified that LRRC3B could inhibit cell cycle progression with downregulation of cyclin D1 and decreased MMP9 expression. In addition, LRRC3B depletion in HBE cells promoted proliferation and invasion. In conclusion, our data suggested that LRRC3B may serve as an important tumor suppressor in NSCLC.

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Acknowledgments

The study was supported by Outstanding Scientific Fund of Shengjing Hospital (No. 201205).

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Authors and Affiliations

  1. Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, China

    Liang Kan, Hui Li, Yi Zhang, Jiahe Wang, Huiyan Niu, Hongfang Jiang, Meng Zhang, Xue Bai & Ping He

  2. Department of Geriatrics, Shengjing Hospital, 36 Sanhao Road, Shenyang, 110004, China

    Ping He

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  1. Liang Kan
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  2. Hui Li
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  7. Meng Zhang
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Correspondence to Ping He.

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Supplementary Figure 1

LRRC3B depletion promotes growth, invasion, cyclin D1 and MMP9. A. Western blot analysis showed that siRNA markedly decreases its levels in HBE cells compared with control. B. MTT assay showed that LRRC3B depletion increased cell growth rate. C. Colony formation assay showed that LRRC3B depletion increased colony number. D. Matrigel invasion assay showed that LRRC3B depletion facilitated HBE cell invasion. E. Western blot analysis showed that LRRC3B depletion slightly upregulated cyclin D1 and MMP9. (GIF 62 kb)

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Kan, L., Li, H., Zhang, Y. et al. LRRC3B is downregulated in non-small-cell lung cancer and inhibits cancer cell proliferation and invasion. Tumor Biol. 37, 1113–1120 (2016). https://doi.org/10.1007/s13277-015-3833-0

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  • DOI: https://doi.org/10.1007/s13277-015-3833-0

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