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Targeting increased copper levels in diethylnitrosamine induced hepatocellular carcinoma cells in rats by epigallocatechin-3-gallate

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Tumor Biology

Abstract

We have earlier elucidated a pathway for the anticancer action of plant polyphenolic compounds against malignant cells involving mobilisation of endogenous copper ions and the consequent prooxidant action. To further confirm our hypothesis in vivo, we induced hepatocellular carcinoma (HCC) in rats by diethylnitrosamine (DEN). We show that in such carcinoma cells, there is a progressive elevation in copper levels at various intervals after DEN administration. Concurrently with increasing copper levels, epigallocatechin-3-gallate (EGCG; a potent anticancer plant polyphenol found in green tea) mediated DNA breakage in malignant cells is also increased. The cell membrane permeable copper chelator neocuproine inhibited the EGCG-mediated cellular DNA degradation, whereas the membrane impermeable chelator bathocuproine was ineffective. Iron and zinc specific chelators desferoxamine mesylate and histidine, respectively, were also ineffective in inhibiting EGCG mediated DNA breakage. Through the use of specific scavengers, the mechanism of DNA breakage was determined to be mediated by reactive oxygen species. In summary, we provide an in vivo evidence of accumulating copper in hepatocellular carcinoma that is targeted by EGCG, leading to its anticancer role in a prooxidant manner. Our findings confirm a novel mechanism of anticancer activity of EGCG in particular and plant derived nutraceuticals in general.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Life Sciences, AMU, Aligarh, India

    Mohd Farhan, Asim Rizvi, Imrana Naseem & S. M. Hadi

  2. Section of Radiobiology, Department of Radiology, The Biomedical Research Tower, The Ohio State University, 460W 12th Avenue, Columbus, OH, USA

    Asim Rizvi

  3. Karmanos Cancer Institute, Wayne State University, 707 HWCRC, 4100 John R St., Detroit, 48201, MI, USA

    Aamir Ahmad

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  1. Mohd Farhan
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Correspondence to Aamir Ahmad.

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Mohd Farhan and Asim Rizvi contributed equally to this work.

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Farhan, M., Rizvi, A., Naseem, I. et al. Targeting increased copper levels in diethylnitrosamine induced hepatocellular carcinoma cells in rats by epigallocatechin-3-gallate. Tumor Biol. 36, 8861–8867 (2015). https://doi.org/10.1007/s13277-015-3649-y

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  • DOI: https://doi.org/10.1007/s13277-015-3649-y

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