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miR-103/107 modulates multidrug resistance in human gastric carcinoma by downregulating Cav-1

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Tumor Biology

Abstract

MicroRNAs (miRNAs) are a class of non-protein-coding small RNAs with the capacity to regulate fundamental biological processes essential for cancer initiation and progression. In the present study, we analyzed miRNA expression levels between multidrug-resistant gastric carcinoma cell line SGC7901/ADR and its parent cell line SGC7901 using a miRNA microarray. MiR-103/107 was downregulated compared with parental SGC7901 cells. Overexpression of miR-103/107 sensitized SGC7901/ADR cells to doxorubicin (DOX), as demonstrated by in vitro and in vivo drug sensitivity assay. We further confirmed that miR-103/107 inhibited P-gp function in gastric cancer SGC7901/ADR cells. Finally, we verified that caveolin-1 (Cav-1), a critical component of lipid rafts, was a target of miR-103/107.

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Acknowledgements

This work was supported by the National Science and Technology Major Project (grant no. 2013ZX09303002), the Chinese National Foundation of National Sciences (grant nos. 81270036, 81372485, 81372546, and 81472193), the Science and Technology project of Liaoning Province (grant no. 2013021057), and the Colleges and Universities of Liaoning Province Outstanding Talent Support Plan (grant no. LR2014023).

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Authors and Affiliations

  1. Department of Medical Oncology, The First Hospital of China Medical University, NO. 155, North Nanjing Street, Heping District, Shenyang, 110001, China

    Ye Zhang, Xiujuan Qu, Ce Li, Yibo Fan, Xiaofang Che, Ying Cai & Yunpeng Liu

  2. Department of Respiratory Medicine, The First Hospital of China Medical University, Shenyang, 110001, China

    Ximing Wang & Xuejun Hu

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  1. Ye Zhang
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  2. Xiujuan Qu
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  3. Ce Li
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  4. Yibo Fan
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  7. Ying Cai
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  8. Xuejun Hu
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  9. Yunpeng Liu
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Correspondence to Xiujuan Qu or Yunpeng Liu.

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Zhang, Y., Qu, X., Li, C. et al. miR-103/107 modulates multidrug resistance in human gastric carcinoma by downregulating Cav-1. Tumor Biol. 36, 2277–2285 (2015). https://doi.org/10.1007/s13277-014-2835-7

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  • DOI: https://doi.org/10.1007/s13277-014-2835-7

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