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Limoniastrum guyonianum aqueous gall extract induces apoptosis in colorectal cancer cells by inhibiting calpain activity

  • Research Article
  • Published:
Tumor Biology

Abstract

Several studies have reported that plant-derived natural products have cancer chemopreventive and chemotherapeutic properties. The aim of the present study was to determine the antiproliferative and pro-apoptotic potential of Limoniastrum guyonianum aqueous gall extract (G extract) on human colorectal cancer BE cell line and, if so, to characterize the mechanism involved. The G extract-induced growth inhibitory effect was associated with an arrest of cell cycle progression in G2/M phase as shown by the cell phase distribution. In addition, G extract promoted in a concentration-dependent manner these cells towards apoptosis as indicated by the presence of cleaved poly(ADP-ribose) polymerase (PARP). In order to characterize the mechanism involved in the antiproliferative and pro-apoptotic signaling pathway activated by G extract, calpain activity and the expression of the cell cycle inhibitor p16INK4A were determined. The present findings indicated that G extract exhibited significant inhibitory activity against calpain and caused a marked and concentration-dependent upregulation of p16INK4A. These effects could be ascribed to the presence of condensed tannins and polyphenols such as epicatechin and epigallocatechin gallate in G extract.

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Abbreviations

G extract:

Aqueous gall extract

DMSO:

Dimethylsulfoxide

TBS:

Tris-buffered saline

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

MALDI-TOF:

Matrix-assisted laser desorption/ionization-time of flight

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Authors and Affiliations

  1. Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisie

    Mounira Krifa, Leila Chekir-Ghedira & Kamel Ghedira

  2. ENSTIB/LERMAB, Epinal, France

    Antonio Pizzi

  3. UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401, Illkirch, France

    Marc Mousli

  4. Laboratoire de Biologie Moléculaire et Cellulaire, Faculté de Médecine Dentaire de Monastir, Rue Avicenne, 5000, Monastir, Tunisie

    Leila Chekir-Ghedira

  5. Aix-Marseille Université, INSERM U911 (CRO2), 27 boulevard Jean Moulin, 13385, Marseille, France

    Ludovic Leloup

  6. Faculté de Médecine Dentaire. Lab. de Biologie Cellulaire et Moléculaire, Rue Avicenne, 5000, Monastir, Tunisie

    Leila Chekir-Ghedira

Authors
  1. Mounira Krifa
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  2. Antonio Pizzi
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  3. Marc Mousli
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  4. Leila Chekir-Ghedira
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  5. Ludovic Leloup
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  6. Kamel Ghedira
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Corresponding author

Correspondence to Leila Chekir-Ghedira.

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Krifa, M., Pizzi, A., Mousli, M. et al. Limoniastrum guyonianum aqueous gall extract induces apoptosis in colorectal cancer cells by inhibiting calpain activity. Tumor Biol. 35, 7877–7885 (2014). https://doi.org/10.1007/s13277-014-1993-y

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  • DOI: https://doi.org/10.1007/s13277-014-1993-y

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