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Hyaluronic acid suppresses the effect of di-(2-ethylhexyl) phthalate in HaCaT keratinocytes

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Abstract

Backgrounds

High molecular weight (HMW) hyaluronic acid (HA) is a major component of the extracellular matrix and functions as an anti-inflammatory and anti-permeability agent. Di-(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer in polymers and it is a common endocrine-disrupting compound that is found in the environment. In this study, we aimed to evaluate the effect of HMW-HA on skin disorders caused by DEHP.

Objectives

We determined the effect of HMW-HA on various cellular processes and pathways in HaCaT keratinocytes treated with DEHP.

Results

The results demonstrated that DEHP-induced cell proliferation significantly increased after HMW-HA treatment in HaCaT keratinocytes. Levels of matrix metalloproteinase (MMP)-2, MMP-9, phospho-nuclear factor-kappa B, cyclooxygenase-2, cleaved-caspase-3, and poly-adenosine diphosphate-ribose polymerase were significantly lower in cells exposed to HMW-HA than in DEHP-treated cells. In addition, DEHP-associated phosphorylation of protein kinase B, extracellular signal-regulated kinase 1/2, and p38 was lower in HMW-HA-treated cells than in DEHP-treated cells.

Conclusion

Therefore, our findings provide evidence that HMW-HA treatment involves the anti-inflammatory cell pathways that prevent or mitigate skin disorders caused by exposure to DEHP.

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Acknowledgements

This study was supported by a National Research Foundation of Korea grant (no. 2017R1D1A1B03034223) founded by the Korean government.

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Authors and Affiliations

  1. Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, 82 Gumi-ro 173 beon-gil Bundang-gu, Seongnam, 13620, South Korea

    Jin Hee Kim

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  1. Jin Hee Kim
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JHK conceived and designed the experiments, performed the experiments, analyzed the data, and wrote the paper.

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Correspondence to Jin Hee Kim.

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JHK states that there are no conflicts of interest to disclose.

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Kim, J.H. Hyaluronic acid suppresses the effect of di-(2-ethylhexyl) phthalate in HaCaT keratinocytes. Mol. Cell. Toxicol. 18, 549–556 (2022). https://doi.org/10.1007/s13273-022-00227-z

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