Abstract
Background
Ultraviolet B (UVB) light can exert toxic effects on the skin by penetrating the epidermal layer and exposing the skin cells, potentially leading to skin disease. Although epidemiological studies have reported associations between UVB and the pathogenesis of skin diseases, few studies have focused on elucidating the underlying mechanisms of skin damage induced by UVB through biological network analysis.
Objective
The present study aimed to explore the potential mechanisms of the toxic effects of UVB on skin and suggested biomarkers by analyzing a signaling network by UVB radiation through a transcriptomic approach.
Results
Through a literature-based network analysis, the oxidative stress and inflammatory response were predicted as major UVB-induced signaling alterations in keratinocytes. UVB light might cause skin cancer, including melanoma and squamous cell carcinoma, through the accumulation of DNA damage and oxidative stress induced by expression changes incorporating CDKN1A, FAS, FOS, MDM2, PLAUR, and TNF.
Conclusion
The analytical approach used in this study suggests that the regulation of gene expression by UVB light leads to skin diseases by changing the cellular processes of keratinocytes. This strategy can be applied to research to suggest mechanisms and biomarkers from a toxicological perspective. Although further functional studies on the modes of action for each factor are required, this study might help develop strategies for exploring UVB light-induced signaling networks through analyzing transcriptomic data.
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Change history
27 June 2022
A Correction to this paper has been published: https://doi.org/10.1007/s13273-022-00242-0
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Acknowledgements
This study was supported by the grant from the Amorepacific Corporation R&I Center, Yongin, Republic of Korea.
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YRS and HJK designed the research study. SJK, HWN and YJ performed overall experimental procedure and analyzed data. DYS reviewed the statistical analysis and also wrote the manuscript. HC reviewed and revised the manuscript.
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Su Ji Kim, Hye-Won Na, Yujin Jang, Dong Yeop Shin, Hyunjung Choi, Hyoung-June Kim, Young Rok Seo declares that they have no conflict of interest.
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Kim, S.J., Na, HW., Jang, Y. et al. Network analysis to understand side effects of UVB on skin through transcriptomic approach. Mol. Cell. Toxicol. 18, 457–467 (2022). https://doi.org/10.1007/s13273-021-00189-8
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DOI: https://doi.org/10.1007/s13273-021-00189-8