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Chlorin e6 and halogen light as a sebostatic photomedicine modulates linoleic acid-induced lipogenesis

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Abstract

Backgrounds

Chlorin e6-mediated photodynamic therapy (Ce6-PDT) showed anti-microbial and anti-inflammatory effects on acne vulgaris model previously. Excess sebum production in sebocytes is a crucial cause of acne. However, information on the effect of Ce6-PDT associated with lipogenesis in sebocytes has not been reported so far.

Methods

The oil red O staining, triglyceride assay and cholesterol assay were used to investigate lipid synthesis in human sebocytes. The expressions of various molecular signals associated with lipogenesis were examined by western blot analysis.

Results

Ce6-PDT reduced the lipid content of cultured sebocytes. The inactivation of Akt, mTOR, Raptor, PPARγ and C/EBPα, as well as the activation of AMPK, were revealed to be the molecular signals associated with sebostatic modulation by Ce6-PDT.

Conclusion

Ce6-PDT using halogen light exerts antilipogenesis in sebocytes and may be utilized for the treatment of acne vulgaris.

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Authors and Affiliations

  1. Department of Medical Science, Soonchunhyang University, 22 SoonChunHyang-ro, Asan, Chungnam, 31538, Republic of Korea

    A-Reum Ryu & Mi-Young Lee

  2. Daegu Cancer Center, Research and Development Unit, DongSung Bio-Pharmaceuticals Co. Ltd., 85 Chembok-ro, Daegu, 41061, Republic of Korea

    Yong-Wan Kim

  3. Department of Medical Biotechnology, Soonchunhyang University, 22 SoonChunHyang-ro, Asan, Chungnam, 31538, Republic of Korea

    Mi-Young Lee

Authors
  1. A-Reum Ryu
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  2. Yong-Wan Kim
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Correspondence to Mi-Young Lee.

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Ryu, AR., Kim, YW. & Lee, MY. Chlorin e6 and halogen light as a sebostatic photomedicine modulates linoleic acid-induced lipogenesis. Mol. Cell. Toxicol. 15, 49–56 (2019). https://doi.org/10.1007/s13273-019-0006-z

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  • DOI: https://doi.org/10.1007/s13273-019-0006-z

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