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Effect of crotonaldehyde on the induction of COX-2 expression in human endothelial cells

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Abstract

Cyclooxygenase-2 (COX-2), an inducible isoform protein, regulates diverse biological actions in vascular pathophysiology. COX-2 is induced in response to numerous stimuli, which results in prostaglandin (PG) production related to inflammation. Crotonaldehyde (CRA) is an extremely toxic α, β-unsaturated aldehyde and a major compound found in cigarette smoke. α, β-Unsaturated aldehyde in cigarette smoke is thought to mediate inflammation and vascular dysfunction. In this study, we evaluated the effect of CRA stimulation on COX-2 expression in human umbilical vein endothelial cells. CRA-stimulated COX-2 induction was accompanied by enhanced p38 phosphorylation and PGE2 generation. However, CRA-induced PGE2 production was reduced by pretreatment with an inhibitor of p38 MAPK. These results demonstrated that in human endothelial cells, CRA-induced COX-2-dependent PGE2 generation was mediated by p38 MAPK, and CRA may play a role in the development of inflammation.

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Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea

    Seung Eun Lee, Cheung-Seog Park, Hyun-Jong Ahn, Jeong-Je Cho & Yong Seek Park

  2. Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, Republic of Korea

    Hye Rim Park, Hyemi Kim & Yeoum Choi

  3. Department of Physiology, School of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea

    Young-Ho Jin

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  1. Seung Eun Lee
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Correspondence to Yong Seek Park.

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Lee, S.E., Park, H.R., Kim, H. et al. Effect of crotonaldehyde on the induction of COX-2 expression in human endothelial cells. Mol. Cell. Toxicol. 13, 345–350 (2017). https://doi.org/10.1007/s13273-017-0038-1

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  • DOI: https://doi.org/10.1007/s13273-017-0038-1

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