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P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma

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Abstract

Background

Hepatocellular carcinoma (HCC) is the most common primary liver malignancy with a high mortality rate. P14.5 is a member of the highly conserved YER057c/YIL051c/YjgF subfamily and is highly expressed in the liver. However, its low expression is associated with carcinogenesis in HCC.

Objective

This study aimed to investigate the role and prognostic significance of P14.5 in HCC.

Methods

The clinical significance of P14.5 in HCC was examined using ONCOMINE, UALCAN, Human Protein Atlas, and Kaplan–Meier plotter. The DNA methylation profile of the P14.5 promoter was examined in 103 HCC and paired precancerous tissues; the HCC cell lines HepG2, MHCC-97L, SMMC-7721, SK-Hep-1, and Huh7; and the normal hepatic cell line HL-7702 via MALDI-TOF mass spectrometry. In addition, in vitro experiments were performed to examine the effects of P14.5 overexpression on the proliferation and migration of SMMC-7721 cells.

Results

Low expression of P14.5 was correlated with shorter overall survival (OS) and disease-free survival (DFS) in HCC. Based on the results of MALDI-TOF mass spectrometry, no difference was observed in the methylation level between HCC cells and normal human hepatic cells and between HCC and paired precancerous tissues. Additionally, P14.5 overexpression promoted the proliferation and inhibited the migration of SMMC7721 cells in vitro.

Conclusions

P14.5 may serve as a prognostic biomarker in HCC and plays a role in the migration and proliferation of HCC cells. Low expression of P14.5 during hepatocarcinogenesis is not attributed to DNA methylation.

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Funding

This work was supported by Natural Science Foundation of Guangxi (No. 2018GXNSFAA138144, No. 2017GXNSFAA198079, and No. 2020GXNSFBA297150). We appreciated Dr Yu Sun for kind help in bioinformatic analysis.

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Author notes

  1. Sa Song and Jian Pan these authors contributed equally.

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Guangxi Medical University, Nanning, 530021, Guangxi, People’s Republic of China

    Sa Song, Yuehuan Xu, Qingniao Zhou & Naixia Chao

  2. Department of Human Anatomy, Guangxi Medical University, Nanning, 530021, Guangxi, People’s Republic of China

    Jian Pan

  3. Department of Histology and Embryology, Guangxi Medical University, Nanning, 530021, Guangxi, People’s Republic of China

    Yaoyao Zhang, Qingmei Zhang, Xiaoxun Xie, Farong Mo & Guorong Luo

  4. Key Laboratory of Biological Molecular Medicine Research (Guangxi Medical University), Education Department of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guangxi, People’s Republic of China

    Sa Song, Yuehuan Xu, Qingniao Zhou & Naixia Chao

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  1. Sa Song
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  2. Jian Pan
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  3. Yaoyao Zhang
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Corresponding author

Correspondence to Naixia Chao.

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Conflict of interest

Author Sa Song, Jian Pan, Yaoyao Zhang, Yuehuan Xu, Qingmei Zhang, Xiaoxun Xie, Qingniao Zhou, Farong Mo, Guorong Luo, and author Naixia Chao declare that they have no conflict of interest.

Ethical approval

This study was approved by the Ethics Committee of the First Affiliated Hospital of Guangxi Medical University (Nanning, China) (No. 20210167). No competing financial interests exist.

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Song, S., Pan, J., Zhang, Y. et al. P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma. Genes Genom 45, 83–91 (2023). https://doi.org/10.1007/s13258-022-01331-z

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