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Correlation of long interspersed element-1 open reading frame 1 and c-Met proto-oncogene protein expression in ovarian cancer

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Abstract

Background

Hypomethylation of long interspersed nuclear element-1 (LINE-1) is closely related to certain cancers and concerns with aggressive tumor behavior. Previously, we reported LINE-1 open reading frame-1 (ORF1) protein level was significantly up-regulated in ovarian cancers compared with normal ovary. Hypomethylation of local LINE-1 sequence has been reported to reactivate MET proto-oncogene in colon cancers and hepatocellular carcinoma. However, the relationship between LINE-1 and c-MET expressions in ovarian cancer is not yet studied.

Method

Here, we analyzed the expression patterns of LINE-1 ORF1 and c-Met protein in ovarian cancer tissue microarrays containing 208 surgical specimens including normal ovary and malignant ovarian cancers.

Results

The expressions of both LINE-1 ORF1 and c-Met protein were significantly increased in ovarian cancers and peaked in early stage of tumor. Other clinical data including age and tumor types were not significantly related with both proteins. Co-relationship between LINE-1 ORF1 and c-Met protein was significant (p = 0.03) but several patients show different expression patterns.

Conclusions

These results propose that LINE-1 ORF1 significantly activates c-Met but not in all cases, suggesting other factors may be involved simultaneously.

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Acknowledgements

This study was supported by Busan Cancer Center Research Grant (2018), Pusan National University Hospital.

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Author notes

  1. Eun-Ji Ko and Young Lim Oh are contributed equally to this work.

Authors and Affiliations

  1. Departments of Parasitology and Genetics, Kosin University College of Medicine, Busan, Republic of Korea

    Eun-Ji Ko, Mee Sun Ock & Hee-Jae Cha

  2. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, Republic of Korea

    Eun-Ji Ko & Heui-Soo Kim

  3. Departments of Obstetrics and Gynecology, Kosin University College of Medicine, Busan, Republic of Korea

    Young Lim Oh & Heung Yeol Kim

  4. Department of Internal Medicine, Kyung Hee University, Seoul, Republic of Korea

    Wan Kyu Eo

  5. Department of Obstetrics and Gynecology, Hallym University College of Medicine, Chuncheon, Republic of Korea

    Hongbae Kim

  6. Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Busan, Republic of Korea

    Ki Hyung Kim

  7. Biomedical Research Institute and Pusan Cancer Center, Pusan National University Hospital, Busan, Republic of Korea

    Ki Hyung Kim

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  1. Eun-Ji Ko
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Correspondence to Ki Hyung Kim or Hee-Jae Cha.

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Author Eun-Ji Ko, author Young Lim Oh, author Heung Yeol Kim, author Wan Kyu Eo, author Hongbae Kim, author Mee Sun Ock, author Heui-Soo Kim, author Ki Hyung Kim, and author Hee-Jae Cha declare that they have no conflict of interest.

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Ko, EJ., Oh, Y.L., Kim, H.Y. et al. Correlation of long interspersed element-1 open reading frame 1 and c-Met proto-oncogene protein expression in ovarian cancer. Genes Genom 41, 1293–1299 (2019). https://doi.org/10.1007/s13258-019-00858-y

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