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Neuroprotective effect of miR-410-3p against sevoflurane anesthesia-induced cognitive dysfunction in rats through PI3K/Akt signaling pathway via targeting C–X–C motif chemokine receptor 5

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Abstract

Background

Postoperative cognitive dysfunction (POCD) is a neurodegenerative disorder with impairment of cognition. Sevoflurane anesthesia has been found to lead to CD and microRNAs (miRNAs) were reported to affect cognitive function. This study investigates the neuroprotective effect against sevoflurane anesthesia-induced CD.

Methods

HE staining was used to detect the pathological change of hippocampal neuron. Morris water maze test was used to analyze latency time, platform crossing and swimming speed. Quantitative real-time PCR (qRT-PCR) and western blotting were performed to examine the mRNA and protein expression of miR-410-3p, IL-6, TNF-α, IL-1β and C–X–C motif chemokine receptor 5 (CXCR5). Dual-luciferase reporter assay was used to detect the relationship between miR-410-3p and CXCR5.

Results

MiR-410-3p was downregulated in sevoflurane anesthesia-induced rats and cells and act as a suppressor in sevoflurane anesthesia-induced hippocampal neuron apoptosis and inflammation. Furthermore, miR-410-3p was identified to bind with CXCR5. Further analysis showed that CXCR5 expression was increased by sevoflurane treatment, whereas was repressed by miR-410-3p overexpression. Moreover, miR-410-3p could inhibit sevoflurane anesthesia-induced hippocampal neuron apoptosis by phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway.

Conclusion

These data indicated that miR-410-3p exhibited its neuroprotective effect on sevoflurane anesthesia-induced CD by targeting CXCR5 via PI3K/Akt signaling pathway. Our study may potentially provide a new light on the pathogenesis and therapeutic method for sevoflurane anesthesia-induced CD.

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All data generated or analyzed during this study are included in this published article.

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Author notes

  1. Rui Su and Ping Sun are contributed equally.

Authors and Affiliations

  1. Department of Anesthesiology, Wuhan Children’s Hospital (Wuhan Meternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430016, Hubei, China

    Rui Su, Chun Feng & Liang Zhong

  2. Department of Anesthesiology, Wuhan Third Hospital-Tongren Hospital of Wuhan University, Wuhan, 430000, Hubei, China

    Ping Sun

  3. Department of Anesthesiology, The Hospital of Shunyi District Beijing, Beijing, 101300, China

    Dianhong Zhang

  4. Department of Anesthesiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, Hubei, China

    Wei Xiao

Authors
  1. Rui Su
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  2. Ping Sun
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  4. Wei Xiao
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  5. Chun Feng
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Contributions

RS and WX conceived and designed the experiments, PS and DHZ analyzed and interpreted the results of the experiments, CF and LZ performed the experiments.

Corresponding author

Correspondence to Wei Xiao.

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Rui Su, Ping Sun, Dianhong Zhang, Wei Xiao, Chun Feng, and Liang Zhong declare that they have no conflict of interest.

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Su, R., Sun, P., Zhang, D. et al. Neuroprotective effect of miR-410-3p against sevoflurane anesthesia-induced cognitive dysfunction in rats through PI3K/Akt signaling pathway via targeting C–X–C motif chemokine receptor 5. Genes Genom 41, 1223–1231 (2019). https://doi.org/10.1007/s13258-019-00851-5

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  • DOI: https://doi.org/10.1007/s13258-019-00851-5

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