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Genome-wide association study of vitamin E using genotyping by sequencing in sesame (Sesamum indicum)

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Abstract

Background

At least eight structurally related forms of vitamin E occur in nature, four tocopherols and four tocotrienols, all of which are potent membrane-soluble antioxidants. In this study, we detected two major isoforms in sesame (Sesamum indicum L.) seed: γ-tocopherol and β-tocotrienol. The objective of this study is to investigate the genetic basis of these vitamin E isoforms.

Methods

We  conducted a genome-wide association study (GWAS) using 5962 genome-wide markers, acquired from 96 core sesame accessions. The GWAS was performed using generalized linear (GLM) and mixed linear (MLM) models.

Results

LG08_6621957, on chromosome 8, was detected as having a significant association with γ-tocopherol in both models. It explained 20.9% of γ-tocopherol variation in sesame. For β-tocotrienol, no significant loci were detected according to the two models, but one locus, SLG03_13104062, explained 17.8% of the phenotypic variation. Based on structure and phylogenetic studies, the 96 accessions were clearly clustered into two subpopulations.

Conclusion

This study on sesame demonstrates and provides an evidence that genotyping by sequencing (GBS) based GWAS can be used to identifying important loci for small growing crops. The significant SNPs or genes could be useful for improving the vitamin E content in sesame breeding programs.

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Acknowledgements

This work was supported by the research grant of the Kongju National University in 2018.

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Authors and Affiliations

  1. Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, 32439, Korea

    Qiang He, Feifei Xu, Myeong-Hyeon Min & Yong-Jin Park

  2. National Key Facility for Crop Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Qiang He

  3. Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China

    Feifei Xu

  4. Center of Crop Breeding On Omics and Artificial Intelligence, Kongju National University, Yesan, 32439, Korea

    Sang-Ho Chu, Kyu-Won Kim & Yong-Jin Park

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  1. Qiang He
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Correspondence to Yong-Jin Park.

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He, Q., Xu, F., Min, MH. et al. Genome-wide association study of vitamin E using genotyping by sequencing in sesame (Sesamum indicum). Genes Genom 41, 1085–1093 (2019). https://doi.org/10.1007/s13258-019-00837-3

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