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The GPX1 Pro198Leu polymorphism in gastric cancer patients with and without Helicobacter pylori infection

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Abstract

Helicobacter pylori infection could induce oxidative stress. Oxidative stress is involved in the pathogenesis of gastric diseases. Glutathione peroxidase 1 (GPX1), is part of the enzymatic antioxidant defense, preventing oxidative damage. The aim of the present study was to assess the association of GPX1 Pro198Leu genotypes with gastric cancer in patients with and without H. pylori infection in a population of Northern Iran. The present case-control study consisted of 50 patients with gastric cancer and 78 cancer-free subjects as controls. Extraction of DNA was performed on bioptic samples and the GPX1 genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of GPX1 Pro/Pro, Pro/Leu and Leu/Leu genotypes in controls were 21.8, 71.8 and 6.4%, respectively. However, in gastric cancer patients, the frequencies of 34, 56 and 10% were observed for Pro/Pro, Pro/Leu and Leu/Leu genotypes, respectively (p = 0.185). In 38 (76%) patients infected with H. pylori, the frequencies of Pro and Leu alleles were 94.7 and 3.3%, respectively. There was a higher frequency of combined genotype of Pro/Leu + Leu/Leu (94.7%) in H. pylori positive patients than that in patients without H. pylori infection (75%, p = 0.047). The presence of this genotype tended to increase the risk of H. pylori related gastric cancer by 5.88–fold (p = 0.069) in our population. Our findings indicated the absence of association between the GPX1 Pro198Leu polymorphism and the risk of gastric cancer in an Iranian population. However, we detected an association between H. pylori related gastric cancer with GPX1 Pro/Leu + Leu/Leu genotype.

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Authors and Affiliations

  1. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad-Taher Moradi

  2. Student Research Committee, Kermanshah University of Medical Science, Kermanshah, Iran

    Kheirollah Yari

  3. Department of Biochemistry, Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Daneshgah Avenue, P.O.Box 67148-69914, Kermanshah, Iran

    Zohreh Rahimi

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  1. Mohammad-Taher Moradi
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  2. Kheirollah Yari
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  3. Zohreh Rahimi
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Correspondence to Zohreh Rahimi.

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Conflict of interest

Mohammad-Taher Moradi declares that he does not have conflict of interest. Kheirollah Yari declares that he does not have conflict of interest. Zohreh Rahimi declares that she does not have conflict of interest.

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All individuals who contributed to this study signed an informed consent in accordance with the Helsinki II declarations. All individuals were informed about the aim and procedures of the research.

Ethical approval

All procedures of the research were accepted and approved by the ethics committee of Kermanshah University of Medical Sciences, Iran.

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Moradi, MT., Yari, K. & Rahimi, Z. The GPX1 Pro198Leu polymorphism in gastric cancer patients with and without Helicobacter pylori infection. Genes Genom 39, 1265–1269 (2017). https://doi.org/10.1007/s13258-017-0594-8

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  • DOI: https://doi.org/10.1007/s13258-017-0594-8

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