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Transcription factor Sp1 is necessary and functional in regulating expression of oncogene ZNF703

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Abstract

Zinc finger protein 703 (ZNF703) is a putative oncogene in patients with the luminal B molecular subtype of breast cancer. Although the exact function of ZNF703 protein remains largely unknown, its expression and regulation have been implicated in several physiological and pathological processes. In the current study, for the first time, we identified and characterized the human ZNF703 gene promoter region. As a means of characterizing the transcription elements required for expression of ZNF703 protein at different stages, we cloned the promoter region of ZNF703 then created chimeric reporter plasmids for use in luciferase assays. A progressive deletion analysis of the ZNF703 gene’s 5′ and 3′ -flanking regions revealed that the core promoter is located in a 256-bp region ranging from nt-539 to nt-283. Next, we examined the effects of site-specific mutations and treatment with mithramycin A to identify the functional Sp1 binding site, which was found to be located in a 447 bp region that ranged from nt-509 to nt-76, displayed the characteristics of a CpG island, and overlapped with the promoter region. In conclusion, our data suggest that ZNF703 transcription is regulated by transcription factor Sp1. This finding should facilitate future studies of the mechanism which regulates expression of this important gene.

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Abbreviations

ANOVA:

Analysis of variance

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Acknowledgements

We thank the Department of Medical Genetics of Sichuan University for generously providing plasmids for creating the pGL3-vector, and also for providing the HEK293T cell line.

Funding

This study was funded by the Sichuan Provincial Education Projects [nos. 15ZB0443].

Author contributions

Xiaolin Liao and Sheng He designed the study, Xiaolin Liao and Yongjie Lu conducted the experiments, Junbao Yang, Tao Kuang, Xiaoli Zhou, Bo Jiang, Huiqun Kang and Yanjun Wang analyzed the data, and Xiaolin Liao and Lilin Jiang wrote the manuscript.

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Authors and Affiliations

  1. Sichuan Nursing Vocational College, Longdu Nanlu #173, Chengdu, 610100, People’s Republic of China

    Xiaolin Liao, Tao Kuang, Lilin Jiang, Yanjun Wang, Huiqun Kang, Bo Jiang, Xiaoli Zhou & Sheng He

  2. Department of Medical Genetics, West China Hospital, Sichuan University, Renmin Nanlu, Section3, #17, Chengdu, 610041, People’s Republic of China

    Xiaolin Liao, Yongjie Lu & Junbao Yang

Authors
  1. Xiaolin Liao
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  2. Yongjie Lu
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  4. Tao Kuang
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  6. Yanjun Wang
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  7. Huiqun Kang
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  8. Bo Jiang
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  9. Xiaoli Zhou
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  10. Sheng He
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Correspondence to Sheng He.

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Conflict of interest

Xiaolin Liao declares that she has no conflict of interest. Yongjie Lu declares that he has no conflict of interest. Junbao Yang declares that he has no conflict of interest. Tao Kuang declares that she has no conflict of interest. Lilin Jiang declares that she has no conflict of interest. Yanjun Wang declares that she has no conflict of interest. Huiqun Kang declares that she has no conflict of interest. Bo Jiang declares that she has no conflict of interest. Xiaoli Zhou declares that she has no conflict of interest. Sheng He declares that he has no conflict of interest.

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This study was approved by ethics committees of West China Hospital, Sichuan University.

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Liao, X., Lu, Y., Yang, J. et al. Transcription factor Sp1 is necessary and functional in regulating expression of oncogene ZNF703 . Genes Genom 39, 1107–1115 (2017). https://doi.org/10.1007/s13258-017-0577-9

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  • DOI: https://doi.org/10.1007/s13258-017-0577-9

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