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Characterization of MSTN/GDF11 gene from shrimp Macrobrachium nipponense and its expression profiles during molt cycle and after eyestalk ablation

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Abstract

Myostatin (MSTN), also known as the growth differentiation factor-8 (GDF-8), belongs to the transforming growth factor-β superfamily. MSTN is a negative regulator of muscle development in vertebrates. However, the transcriptional regulation of MSTN in freshwater crustaceans is still unclear. In this study, a cDNA encoding for MSTN/GDF11 (Mn-MSTN/GDF11) was cloned from the oriental freshwater shrimp, Macrobrachium nipponense. The full-length cDNA sequence of Mn-MSTN/GDF11 was composed of 1733 nucleotides, including a 5′ UTR of 119 nucleotides, an open read frame of 1359 nucleotides, and a 3′ UTR of 255 nucleotides. The predicted peptide of Mn-MSTN/GDF11 has 452 amino acids with the conserved RXXR cleavage site and nine cysteines. Tissue-specific expression pattern showed that Mn-MSTN/GDF11 is mainly expressed in abdominal muscle. During the course of embryonic development, expression level of Mn-MSTN/GDF11 could be detected after gastrul stage, and reached at the highest level at embryonized-zoea stage. During molt cycle, expression level of Mn-MSTN/GDF11 mRNA was up-regulated significantly at early postmoult stage, but down-regulated gradually in the following molt stages. In the period of 14 days after eyestalk ablation, Mn-MSTN/GDF11 transcripts were significantly decreased in abdominal muscle and heart, but increased in thoracic muscle. The results of this study indicated that Mn-MSTN/GDF11 may play a role in molt cycle and be regulated by hormone secreted in eyestalk.

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Acknowledgments

This study was supported by the Public Project of Zhejiang Province, P. R. China (No. 2013C32068) and the Foundation of Zhejiang Education Committee of China (Y201225243).

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The authors declare no conflict of interest in the current study.

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    Authors and Affiliations

    1. Department of Biology, College of Life Science, Shaoxing University, Shaoxing, 312000, Zhejiang, China

      Wenying Shen, Gang Ren, Yaorong Zhu & Xiaodong Zhang

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    1. Wenying Shen
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    2. Gang Ren
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    4. Xiaodong Zhang
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    Correspondence to Wenying Shen.

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    Wenying Shen and Gang Ren have contributed equally to this work.

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    Shen, W., Ren, G., Zhu, Y. et al. Characterization of MSTN/GDF11 gene from shrimp Macrobrachium nipponense and its expression profiles during molt cycle and after eyestalk ablation. Genes Genom 37, 441–449 (2015). https://doi.org/10.1007/s13258-015-0273-6

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