Skip to main content
SpringerLink
Log in

Heliocoverpa armigera single nucleocapsid nucleopolyhedrovirus ORF52 is a ChaB homologous gene involved in per os infection

  • Research Article
  • Published:
Genes & Genomics Aims and scope Submit manuscript

Abstract

The baculovirus ChaB proteins are conserved in all completely sequenced Lepidopteran NPVs and are annotated as putative DNA binding proteins. In our previous study, ORF51, a ChaB homologue from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was found to be involved in budded virus production and DNA replication. In the present study, we characterized ORF52 (Ha52), the other ChaB homologous gene in HearNPV. 5′-RACE revealed that Ha52 was transcribed from a conventional late promoter transcriptional initiator motif (TAAG) located 25 nucleotides upstream of ATG. Expression analysis demonstrated that HA52 protein was expressed from 48 to 96 h post infection. Western blot analysis of virions from both budded viruses (BVs) and occlusion-derived viruses (ODVs) indicated that HA52 was a structural component of nucleocapsid from ODV. To study the function of Ha52 in the life cycle of HearNPV, Ha52-knockout and Ha52-restored viruses were generated by the Bac-to-Bac system. Growth curve analyses showed that the level of BV production in cells infected with Ha52 null virus was similar to those infected with wild-type bacmid derived virus. However, bioassay showed that deletion of Ha52 significantly decreased the per os infectivity of HearNPV. Taken together, our results indicated that, unlike the previously characterized baculovirual ChaB genes, Ha52 may be involved in HearNPV per os infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Braunagel SC, Summers MD (1994) Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid profiles. Virology 202:315–332

    Article  CAS  PubMed  Google Scholar 

  • Braunagel SC, Russell WK, Rosas-Acosta G, Russell DH, Summers MD (2003) Determination of the protein composition of the occlusion-derived virus of Autographa californica nucleopolyhedrovirus. Proc Natl Acad Sci USA 100:9797–9802

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Chen X, IJkel WF, Tarchini R, Sun X, Sandbrink H, Wang H, Peters S, Zuidema D, Lankhorst RK, Vlak JM et al (2001) The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J Gen Virol 82:241–257

    Article  CAS  PubMed  Google Scholar 

  • Crooks GE, Hon G, Chandonia JM, Brenner SE (2004) WebLogo: a sequence logo generator. Genome Res 14:1188–1190

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dandekar T, Snel B, Huynen M, Bork P (1998) Conservation of gene order: a fingerprint of proteins that physically interact. Trends Biochem Sci 23:324–328

    Article  CAS  PubMed  Google Scholar 

  • Datsenko KA, Wendell B (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97:6640–6645

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Deng F, Wang R, Fang M, Jiang Y, Xu X, Wang H, Chen X, Arif BM, Guo L, Wang H et al (2007) Proteomics analysis of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, HA44 and HA10. J Virol 81:9377–9385

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Funk CJ, Braunagel SC, Rohrmann GF (1997) Regulation of baculovirus late and very late gene expression. Plenum, New York

    Google Scholar 

  • Huang H, Wang M, Deng F, Wang H, Hu Z (2012) ORF85 of HearNPV encodes the per os infectivity factor 4 (PIF4) and is essential for the formation of the PIFcomplex. Virology 427(2):217–223

    Article  CAS  PubMed  Google Scholar 

  • Ivey DM, Guffanti AA, Zemsky J, Pinner E, Karpel R, Padan E, Schuldiner S, Krulwich TA (1993) Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporterdeficient strains by the overexpressed gene. J Biol Chem 268:11296–11303

    CAS  PubMed  Google Scholar 

  • Jehle JA, Blissard GW, Bonning BC, Cory JS, Herniou EA, Rohrmann GF, Theilmann DA, Thiem SM, Vlak JM (2006) On the classification and nomenclature of baculovirus: a proposal for revision. Arch Virol 151:1257–1266

    Article  CAS  PubMed  Google Scholar 

  • Li L, Li Z, Chen W, Liu C, Huang H, Yang K, Pang Y (2006a) Characterization of Spodoptera exigua multicapsid nucleopolyhedrovirus ORF100 and ORF101, two homologues of E. coli ChaB. Virus Res 121:42–50

    Article  CAS  PubMed  Google Scholar 

  • Li Z, Li L, Yu H, Li S, Pang Y (2006b) Characterization of two homologues of ChaB in Spodoptera litura multicapsid nucleopolyhedrovirus. Gene 372:33–43

    Article  CAS  PubMed  Google Scholar 

  • McIntosh AH, Christian PD, Grasela JJ (1999) The establishment of heliothine cell lines and their susceptibility to two baculovirus. In Vitro Cell Dev Biol 35:94–97

    Article  CAS  Google Scholar 

  • Noble ME, Endicott JA, Brown NR, Johnson LN (1997) The cyclin box fold: protein recognition in cell-cycle and transcription control. Trends Biochem Sci 22:482–487

    Article  CAS  PubMed  Google Scholar 

  • O’Reilly DR, Miller LK, Luchow VA (1992) Baculovirus expression vectors: a laboratory manual. Freeman, New York

    Google Scholar 

  • Osborne MJ, Siddiqui N, Iannuzzi P, Gehring K (2004) The solution structure of ChaB, a putative membrane ion antiporter regulator from Escherichia coli. BMC Struct Biol 4:9

    Article  PubMed Central  PubMed  Google Scholar 

  • Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M et al (1996) A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7–28.0 min region on the linkage map (supplement). DNA Res 3:211–223

    Article  CAS  PubMed  Google Scholar 

  • Peng K, van Lent JW, Boeren S, Fang M, Theilmann DA, Erlandson MA, Vlak JM, van Oers MM (2012) Characterization of novel components of the baculovirus per os infectivity factor complex. J Virol 86:4981–4988

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Robertson JL, Preisler HK (1992) Pesticide bioassays with arthropods. CRC Press, Baton Rouge

    Google Scholar 

  • Slack J, Arif BM (2007) The baculoviruses occlusion-derived virus: virion structure and function. Adv Virus Res 69:99–165

    Article  CAS  PubMed  Google Scholar 

  • Wu CP, Huang YJ, Wang JY, Wu YL, Lo HR, Wang JC, Chao YC (2010) Autographa californica multiple nucleopolyhedrovirus LEF-2 is a capsid protein required for amplification but not initiation of viral DNA replication. J Virol 84:5015–5024

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Xiang X, Chen L, Guo A, Yu S, Yang R, Wu X (2011) The Bombyx mori nucleopolyhedrovirus (BmNPV) ODV-E56 envelope protein is also a per os infectivity factor. Virus Res 155:69–75

    Article  CAS  PubMed  Google Scholar 

  • Zheng F, Huang Y, Long G, Sun X, Wang HZ (2011) Heliocoverpa armigera single nucleocapsid nucleopolyhedrovirus ORF51 is a ChaB homologous gene involved in budded virus production and DNA replication. Virus Res 155:203–212

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from the education department of Liaoning province (Project No. L2012008) and youth fund of Liaoning University (Project No. 2010LDQN05). We thank Dr. Zhihong Hu for the anti-HearNPV VP39 and anti-E18 antibodies, Dr. Hanzhong Wang for technical support, and Dr Alan K Chang for revising the language of the manuscript.

Conflict of interest

The authors have declared no conflict of interest.

Author information

Authors and Affiliations

  1. Key Laboratory of Animal Resource and Epidemic Disease Prevention, Life Science School of Liaoning University, Shenyang, 110036, China

    Chunyu Zhu, Hongsheng Liu, Maosheng Zhang, Shuqin Xiong & Fangliang Zheng

  2. Henan University of TCM, Zhengzhou, 450046, China

    Sugai Yin

  3. Shenyang Institute of Standardization (SYIS), Shenyang, 110021, China

    Yan Liu

Authors
  1. Chunyu Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sugai Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hongsheng Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maosheng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shuqin Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fangliang Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fangliang Zheng.

Additional information

Chunyu Zhu and Sugai Yin have contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhu, C., Yin, S., Liu, H. et al. Heliocoverpa armigera single nucleocapsid nucleopolyhedrovirus ORF52 is a ChaB homologous gene involved in per os infection. Genes Genom 36, 843–851 (2014). https://doi.org/10.1007/s13258-014-0222-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13258-014-0222-9

Keywords

Search

Navigation