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Genetic diversity and phylogeny of Termitomyces spp. occurring in Assam, India

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Abstract

Termitomyces are found associated with termites and show an exo-symbiotic relationship with termites. Both termites and Termitomyces are important in forest ecosystems for wood degradation. The morphological diversity of Termitomyces mushrooms as observed in Assam is high similar to other forest-rich regions of India. But the genetic diversity and species diversity from the region is not yet documented well. In the present study, DAMD and ISSR markers were applied for genetic diversity estimation and nrDNA ITS marker was applied for the phylogenetic study of the Termitomyces mushrooms collected from different localities of Assam. Besides, morphological characters were compared to the existing species monographs to identify the species. Based on morpho-molecular studies, the species identified are T. heimii, T. microcarpus, and T. eurrhizus. Five morphotypes of Termitomyces different from the existing species descriptions are recorded and are also sub-clustered distinctly and distantly in a phylogenetic tree generated based on nrDNA ITS sequences. The presence of novel species of Termitomyces is very much likely in the region which needs further taxonomic engagements to establish the neo species through valid publication.

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Acknowledgements

The authors thank the Department of Botany, Gauhati University, Guwahati and the Plant Systematics & Herbarium Division, CSIR-NBRI, Lucknow for laboratory facilities. A special thanks to late Prof. D.K. Jha who inspired us to work on Termitomyces. Our sincere thanks to all those who directly or indirectly helped us during the collection of Termitomyces samples in the field.

Funding

This research work was funded by the Department of Biotechnology, Govt. of India under the DBT Twin. Scheme for NER [Project No. BT/PR16230/NER/95/101/2015]

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  1. Microbiology and Molecular Systematics Laboratory, Department of Botany, Gauhati University, Guwahati, 781014, India

    Lakshmi Rupa Das, Deepjyoti Das & Diganta Narzary

  2. Plant Diversity, Systematics and Herbarium Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Vandana Tiwari & Tikam S. Rana

  3. Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Brahma N. Singh

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LRD and DD collected the samples and did morphological studies, DNA isolation, and molecular studies; VT did molecular profiling for genetic diversity study. DN, BNS and TSR supervised the experimental work. Manuscript was drafted by LRD, and finalized by DN and TSR.

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Das, L.R., Tiwari, V., Das, D. et al. Genetic diversity and phylogeny of Termitomyces spp. occurring in Assam, India. Nucleus (2023). https://doi.org/10.1007/s13237-023-00434-6

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