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A Controlled Transcription-Driven Light-Up Aptamer Amplification for Nucleoside Triphosphate Detection

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Abstract

In this study, we introduced a simple method for detecting nucleoside triphosphates (NTPs) using controlled transcription-driven light-up aptamer amplification. Based on the concentration of target NTP, the light-up aptamer was amplified using a DNA template encoding the Broccoli aptamer sequence through an incomplete transcription mixture (without each NTP). The Broccoli aptamer associated with DFHBI-1T produced significantly enhanced fluorescence signals that could sensitively detect NTPs in a label-free manner. In addition, the proposed assay was useful for quantitatively detecting NTPs in serum. Thus, we expect that this method has great potential for NTP analysis in bioassays and biological researches.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This research was supported by the National Research Foundation of Korea (NRF-2021R1C1C1009105) and the National Research Council of Science and Technology (NST) (CRC22021-500).

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Author notes

  1. Deok-Gyu Lee and Hye Jin Lim contributed equally to this work.

Authors and Affiliations

  1. Critical Diseases Diagnostics Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea

    Deok-Gyu Lee, Ha-Yeong Lee & Ju-Young Byun

  2. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, 34113, Republic of Korea

    Ha-Yeong Lee, Ju-Young Byun & Yong-Beom Shin

  3. Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305764, Korea

    Deok-Gyu Lee & Dong-Myung Kim

  4. Apteasy MJ Inc., 333 Cheomdangwagi-ro, Technopark, Gwangju, 61008, Korea

    Kyung-Ho Lee

  5. Department of Chemical Engineering, Stanford University, 443 Via Ortega, Stanford, CA, 94305, USA

    Hye-Jin Lim

  6. BioNano Health Guard Research Center, Daejeon, 34141, Republic of Korea

    Yong-Beom Shin

  7. Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea

    Yong-Beom Shin

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  1. Deok-Gyu Lee
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Correspondence to Kyung-Ho Lee, Ju-Young Byun or Yong-Beom Shin.

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Lee, DG., Lim, HJ., Lee, HY. et al. A Controlled Transcription-Driven Light-Up Aptamer Amplification for Nucleoside Triphosphate Detection. BioChip J 17, 487–495 (2023). https://doi.org/10.1007/s13206-023-00124-0

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  • DOI: https://doi.org/10.1007/s13206-023-00124-0

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