Abstract
Intestinal microbiota disorder was associated with constipation. This study investigated the microbiota–gut–brain axis and oxidative stress mediated by intestinal mucosal microbiota in mice with spleen deficiency constipation. The Kunming mice were randomly divided into the control (MC) group and the constipation (MM) group. The spleen deficiency constipation model was established by gavage with Folium sennae decoction and controlled diet and water intake. The body weight, spleen and thymus index, 5-Hydroxytryptamine (5-HT) and Superoxide Dismutase (SOD) content were significantly lower in the MM group than the MC group, the content of vasoactive intestinal peptide (VIP) and malondialdehyde (MDA) content were significantly higher than the MC group. The Alpha diversity of intestinal mucosal bacteria was not changed but beta diversity was changed in mice with spleen deficiency constipation. Compared to the MC group, the relative abundance of Proteobacteria was an upward trend and the Firmicutes/Bacteroidota (F/B) value was a downward trend in the MM group. There was a significant difference in the characteristic microbiota between the two groups. In the MM group, Brevinema, Akkermansia, Parasutterella, Faecalibaculum, Aeromonas, Sphingobium, Actinobacillus, and other pathogenic bacteria were enriched. Meanwhile, there was a certain relationship between the microbiota and gastrointestinal neuropeptide and oxidative stress indicators. The community structure of intestinal mucosal bacteria in mice with spleen deficiency constipation was changed, which was characterized by the reduction of F/B value and enrichment of Proteobacteria. Microbiota–gut–brain axis may be important for spleen deficiency constipation.
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Data availability
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request. The data presented in the study are deposited in the NCBI repository, accession number PRJNA878625.
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Acknowledgements
We thank the Natural Science Foundation of Hunan Province (No. 2022JJ40332) and the Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine (4901-020000200207) for the financial support of this study.
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This research was financially supported by the Natural Science Foundation of Hunan Province (No. 2022JJ40332) and the Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine (4901-020000200207).
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XY: data analysis and writing the original draft. PJ and ND: review and editing. KZ and XY: performing animal experiments. XP and ZT: project administration, review, and funding acquisition. All the authors contributed to the article and approved the submitted version.
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Animal experiments were conducted under animal protocols approved by the Animal Ethics and Welfare Committee of the Hunan University of Chinese Medicine (protocol number: LL2022052505). All animal work was carried out following accordance with the guidelines of the Institutional Animal Care and Use Committee of Hunan University of Chinese Medicine. This study was carried out in compliance with the ARRIVE guidelines.
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Yi, X., Zhou, K., Jiang, P. et al. Brain-bacteria-gut axis and oxidative stress mediated by intestinal mucosal microbiota might be an important mechanism for constipation in mice. 3 Biotech 13, 192 (2023). https://doi.org/10.1007/s13205-023-03580-5
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DOI: https://doi.org/10.1007/s13205-023-03580-5