Abstract
In the present study, 36 Asian rice cultivars/landraces were evaluated against M. graminicola under in vitro conditions using soilless Pluronic gel medium. The cultivars/genotypes Phule Radha, EK 70, LK 248 and Khalibagh showed significantly reduced nematode infection, endoparasitic development, and derived multiplication factor indicating the presence of resistance, while Halvi Sal 17 was found to be most susceptible. Performance of selected genotypes showing resistance/susceptibility under in vitro conditions was further confirmed in soil which also revealed Phule Radha to be highly resistant and Halvi Sal 17 as the most susceptible genotype. Further, expression profile of plant defense responsive genes related to MAPK pathway, phytohormones, PR-proteins and callose and lignin synthesis were quantified in Phule Radha (the most resistant) and Halvi Sal 17 (the most susceptible) at 2 and 6 days post nematode inoculation. Significant upregulated expression of several defensive genes was observed in the resistant cultivar Phule Radha in contrast to insignificant expression in the susceptible varieties. The resistant genotype identified in the present study will be highly promising for resistance breeding in rice against M. graminicola.
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Abbreviations
- PPNs:
-
Plant parasitic nematodes
- SA:
-
Salicylic acid
- JA:
-
Jasmonic acid
- ET:
-
Ethylene
- J2s:
-
Second stage juvenile
- MF:
-
Multiplication factor
- MAPK:
-
Mitogen activated protein kinase
- PB:
-
Pusa Basmati 1121
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Acknowledgments
Authors acknowledge funding from Department of Biotechnology (DBT) COE project BT/PR-18924/COE/34/48/2017. The role of funding body does not include data analysis, interpretation and manuscript writing. The Ph.D. student BH acknowledges the Post Graduate School, Indian Council of Agricultural Research (ICAR)-Indian Agricultural Research Institute (IARI), New Delhi for research fellowship.
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UR and BH conceived and designed the study. BH and DS performed the experiments. BH analysed the data. SK supplied the seeds on requirement. BH, VP and UR wrote and revised the manuscript.
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Hatzade, B., Singh, D., Phani, V. et al. Profiling of defense responsive pathway regulatory genes in Asian rice (Oryza sativa) against infection of Meloidogyne graminicola (Nematoda:Meloidogynidae). 3 Biotech 10, 60 (2020). https://doi.org/10.1007/s13205-020-2055-3
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DOI: https://doi.org/10.1007/s13205-020-2055-3