Abstract
Staphylococcus aureus and Staphylococcus lugdunensis are often associated with pathogenic biofilms ranging from superficial mucosal to life-threatening systemic infections. Recent studies have reported that chelerythrine (CHE) displays antimicrobial activities against a few microorganisms, but its effects on dual-species biofilms of S. aureus and S. lugdunensis have never been reported. The purpose of this study was to investigate how dual-species biofilms of S. aureus and S. lugdunensis respond when challenged with CHE. Minimum inhibitory concentration (MIC) of CHE against planktic cells in dual-species culture was 8 μg/mL. CHE also suppressed dual-species biofilm formation at minimal biofilm inhibitory concentration (MBIC90, 4 μg/mL). Further, confocal laser scanning microscope (CLSM) using five fluorescent dyes revealed the dose-dependent reduction of the levels of three key biofilm matrix components, and reduced tolerance to gatifloxacin, of biofilms exposed to CHE. Moreover, CHE efficiently eradicated preformed dual-species biofilms at minimal biofilm eradication concentration (MBEC, 256 μg/mL). Hence, CHE has the potential to address biofilm infections of clinical course and other biofilm-related diseases caused by S. aureus and S. lugdunensis.
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Acknowledgements
This study was funded by the Key Research and Development Project of Shaanxi Province (2019JM-184), and the Industry Cultivation Project of Education Department of Shaanxi Provincial Government (18JC006, 18JC007).
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Design of the work was performed by WQ. Acquisition of data, analysis and interpretation, drafting and critical revision, approval of the final version and agreement to be accountable for the work were performed by ZS, YF, MY, TW, and YL.
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Qian, W., Sun, Z., Fu, Y. et al. Efficacy of chelerythrine against dual-species biofilms of Staphylococcus aureus and Staphylococcus lugdunensis. 3 Biotech 10, 427 (2020). https://doi.org/10.1007/s13205-020-02401-3
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DOI: https://doi.org/10.1007/s13205-020-02401-3