Abstract
The present study explored the immobilization of laccase onto iron magnetic nanoparticles (MNPs) to enhance its enzymatic properties and applications. The immobilization process was optimized using Box–Behnken design (BBD). BBD showed significance towards the quadratic model with experimental data. Maximum laccase activity recovery (99%) of the predicted model was observed at 0.75 mg/mL of laccase concentration, 200 mg/mL of MNPs, 0.3% cross linking with carbodiimide, and 3 h of cross-linking time. The magnetization activity of MNPs (8 emu/g) and the immobilized laccase with MNPs (4 emu/g) was analyzed using vibrating sample magnetometer (VSM). Maximum activity of immobilized laccase was observed at pH 7.0 and 55 °C. The immobilized laccase has greater stability (100 h) and significant chlorpyrifos (pesticide) degradation activity. High-performance liquid chromatography (HPLC) results confirmed the degraded metabolic products of chlorpyrifos. In all, the immobilized laccase was superior to free laccase, showing promising structural and application characteristics.
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Acknowledgements
This research is partially supported by the PG & Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), and the Department of Science and Technology, Government of India (DST-FIST sponsored-Ref. No. SR/ FST/College-232/2014). This research was supported by Kyungpook National University Bokhyeon Research Fund, 2017. This study was supported by Researchers Supporting Project number (RSP-2020/144), King Saud University, Riyadh, Saudi Arabia.
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Srinivasan, P., Selvankumar, T., Paray, B.A. et al. Chlorpyrifos degradation efficiency of Bacillus sp. laccase immobilized on iron magnetic nanoparticles. 3 Biotech 10, 366 (2020). https://doi.org/10.1007/s13205-020-02363-6
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DOI: https://doi.org/10.1007/s13205-020-02363-6