Abstract
Different desert truffles, collected from Algerian Saharan soils, were identified and their capacity to produce bioactive substances with antimicrobial activity was analyzed. Based on morphological characterization using Melzer’s reagent staining, the collected strains were identified as Terfezia arenaria. The bioactive substances from T. arenaria were extracted using the following techniques: maceration with methanol and Soxhlet with dichloromethane. The former led to a yield much higher than that of the latter (i.e., 15% and 0.48%, respectively). Both extracts presented antifungal activities against all the tested strains (i.e., A. niger, Penicillium sp., and C. albicans). However, the dichloromethane extracts showed much higher antibacterial activities against all the tested bacteria (i.e., S. aureus, E. faecalis, E. coli, and P. aeruginosa) than the methanol extracts. The thin layer chromatography of both extracts confirmed the presence of polyphenols and flavonoids.
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References
Attia WY, El-Naggar RE, Bawadekji A, Al Ali M (2018) Evaluation of some in vitro anti-carcinogenic activities of polysaccharides extracted from Ascomata of the desert truffle Terfezia claveryi Chatin. J Appl Environ Biol Sci 8:152–159
Bassah G (1999) Contribution à l’étude de la symbiose mycorhiziennes entre deux espèces de Terfez: Terfezia claveryi, Terfezia boudieri avec le pin d’Alpe (Pinus halepensis Mill) dans la région du Djelfa. M.Sc. Thesis. Institut National Agronomique El-Harach, Algeria
Bouchet R, Siebert E (1999) Proton conduction in acid doped polybenzimidazole. Solid State Ionics 118:287–299
Bradai L (2006) Contribution à l’étude bioécologique de la truffe blanche de désert (Terfezia sp) cas de la région de Oued Mya (Ouargla). M.Sc. Thesis. University Kasdi Merbah Ouargla, Algeria
Chatin A (1892) La Truffe. Botanique de la Truffe et des plantes Truffières, J.E. Baillière et fils, Paris
Chebil L, Humeau C, Anthoni J, Dehez F, Engasser JM, Ghoul M (2007) Solubility of flavonoids in organic solvents. J Chem Eng Data 52:1552–1556
Cole GM, Lim GP, Yang F, Teter B, Begum A, Ma Q, Harris-White MC, Frautschy A (2005) Prevention of Alzheimer’s disease: omega-3 fatty acid and phenolic antioxidant interventions. Neurobiol Aging 26:S133–S136
Dib-Bellahouel S, Fortas Z (2014) Activity of the desert truffle Terfezia boudieri Chatin, against associated soil microflora. Afr J Microbiol Res 8:3008–3016
Gouzi F, Prefaut C, Abdellaoui A, Vuillemin A, Molinari N, Ninot G, Hayot M (2011) Evidence of an early physical activity reduction in chronic obstructive pulmonary disease patients. Arch Phys Med Rehabil 92:1611–1617
Hamza A, Zouari N, Zouari S, Jdir H, Zaidi S, Gtari M, Neffati M (2016) Nutraceutical potential, antioxidant and antibacterial activities of Terfezia boudieri Chatin, a wild edible desert truffle from Tunisia arid zone. Arab J Chem 9:383–389
Josserand A (1983) Apport de l’immunofluorescence à l’étude écologique des germes nitrifiants (genre Nitrobacter). Ph.D. Thesis, University of Lyon, France
Khanaqa A (2006) Truffle production in the Kingdom of Saudi Arabia-potential and limitation. J Appl Bot Food Qual 80:14–18
Lamaison JL, Petitjean-Freyet C, Carnat A (1991) Lamiacees medicinales à proprietes antioxydantes, sources potentielles d’acide rosmarinique. Pharm Acta Helv 66:185–188
Mansar-Benhamza L, Djerrou Z, Pacha H (2013) Evaluation of anti-hyperglycemic activity and side effects of Erythraea centaurium (L) Pers in rats. Afr J Biotechnol 12:6980–6985
Nazck M, Shahidi F (2004) Extraction and analysis of phenolics in food. J Chromatogr A 1054:95–111
Neggaz S, Fortas Z, Chenni M, El Abed D, Ramli B, Kambouche N (2015) In vitro evaluation of antioxidant, antibacterial and antifungal activities of Terfezia claveryi. Chatin Phytothérapie 16:20–26
Riboli E, Norat T (2003) Epidemiologic evidence of the protective effect of fruit and vegetables on cancer risk. Am J Clin Nutr 78:559–569
Seghiri R, Boumaza O, Mekkiou R, Benayache S, Mosset P, Quintana J, Benayache F (2009) A flavonoid with cytotoxic activity and other constituents from Centaurea africana. Phytochem Lett 2:114–118
Tadja A (1996) Etude écologique de deux espèces de Terfezia du sud Oued Algérie Essai de leur mycorhization sur trois espèces céréalières. M.Sc. Thesis Institut National Agronomique El-Harrach, Algeria
Trappe JM (1979) The orders, families and genera of Hypogeous ascomycotina (truffles and their relatives). Mycotaxon 9:297–340
Wasser S (2010) Medicinal mushroom science: history, current status, future trends, and unsolved problems. Int J Med Mushrooms 12:1–16
Waterman PG (1994) Analysis of phenolic plant metabolites. Blackwell Scientific Publications, Oxford
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Authors acknowledge the contribution of Dr. Peter Elias Kidibule of Universidad Autónoma de Madrid (UAM), Spain, as well as volunteers for completion of this study.
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Harir, M., Bendif, H., Yahiaoui, M. et al. Evaluation of antimicrobial activity of Terfezia arenaria extracts collected from Saharan desert against bacteria and filamentous fungi. 3 Biotech 9, 281 (2019). https://doi.org/10.1007/s13205-019-1816-3
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DOI: https://doi.org/10.1007/s13205-019-1816-3