Abstract
Here, we report on the isolation of bacterial isolates from Himalayan niches, which produced extracellular l-asparaginase with low/no glutaminase activity. From the 235 isolates, 85 asparaginase positive bacterial isolates were identified by qualitative screening using optimized chromogenic dyes assay. Optimized concentration of different dyes revealed maximum color visualization in phenol red (0.003%). The diversity analysis of asparaginase positive isolates revealed that Proteobacteria (83%) are the most dominant, followed by Actinobacteria (12%), Firmicutes (3%), and Bacteriodetes (2%). Eleven isolates, which represented seven Pseudomonas species, one species each of the genus Arthrobacter, Janthinobacterium, Lelliottia, and Rahnella, were selected for further studies based on highest zone ratio and novel aspects for l-asparaginase production. Of these, five isolates, namely, Pseudomonas sp. PCH133, Pseudomonas sp. PCH146, Pseudomonas sp. PCH182, Rahnella sp. PCH162, and Arthrobacter sp. PCH138, produced l-asparaginase without glutaminase activity after 55 h of growth with the former isolate showing the highest l-asparaginase activity (1.67 U/ml). Interestingly, this is the first report of l-asparaginase production by members of the genera Janthinobacterium, Rahnella, and Lelliottia.
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Acknowledgements
Authors duly acknowledge the technical assistance provided by Anil Kumar Chaudhary for 16S rDNA sequencing. This manuscript represents CSIR-IHBT Communication No. 4293.
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DS duly acknowledge the financial support from CSIR, Govt. of India for CSIR-Fast Track Translation (FTT) project (33/BS/FTT/2016-PPD) and CSIR Network Project CeHAB (BSC0209).
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Kumar, V., Kumar, S., Darnal, S. et al. Optimized chromogenic dyes-based identification and quantitative evaluation of bacterial l-asparaginase with low/no glutaminase activity bioprospected from pristine niches in Indian trans-Himalaya. 3 Biotech 9, 275 (2019). https://doi.org/10.1007/s13205-019-1810-9
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DOI: https://doi.org/10.1007/s13205-019-1810-9