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Fabrication and characterization of chitosan nanoparticles and collagen-loaded polyurethane nanocomposite membrane coated with heparin for atrial septal defect (ASD) closure

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Abstract

Atrial septal defect (ASD) constitutes 30–40% of all congenital heart diseases in adults. The most common complications in the treatment of ASD are embolization of the device and thrombosis formation. In this research, an occluding patch was developed for ASD treatment using a well-known textile technology called electrospinning. For the first time, a cardiovascular occluding patch was fabricated using medical grade polyurethane (PU) loaded with bioactive agents namely chitosan nanoparticles (Cn) and collagen (Co) which is then coated with heparin (Hp). Fourier transform infrared spectrum showed characteristic vibrations of several active constituents and changes in the absorbance due to the inclusion of active ingredients in the patch. The contact angle analysis demonstrated no significant decrease in contact angle compared to the control and the composite patches. The structure of the electrospun nanocomposite (PUCnCoHp) was examined through scanning electron microscopy. A decrease in nanofiber diameter between control PU and PUCnCoHp nanocomposite was observed. Water uptake was found to be decreased for the PUCnCoHp nanocomposite against the control. The hemocompatibility properties of the PUCnCoHp ASD occluding patch was inferred through in vitro hemocompatibility tests like activated partial thromboplastin time (APTT), prothrombin time (PT) and hemolysis assay. It was found that the PT and APTT time was significantly prolonged for the fabricated PUCnCoHp ASD occluding patch compared to the control. Likewise, the hemolysis percentage was also decreased for the PUCnCoHp ASD patch against the control. In conclusion, the developed PUCnCoHp patch demonstrates potential properties to be used for ASD occlusion.

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Acknowledgements

This work was supported partly by the Ministry of Higher Education Malaysia with the Grant Vot number: Q.J130000.2545.12H80.

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Authors and Affiliations

  1. Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia

    Eva Kaiser & Eko Supriyanto

  2. Ilmenau Institute of Biomedical Engineering and Computer Science, Technical University of Ilmenau, 98693, Ilmenau, Germany

    Eva Kaiser

  3. Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Saravana Kumar Jaganathan

  4. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Saravana Kumar Jaganathan

  5. IJN-UTM Cardiovascular Engineering Centre, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81300, Skudai, Johor, Malaysia

    Saravana Kumar Jaganathan

  6. Department of Chemistry, Bharat Institute of Higher Education and Research, Bharat University, Chennai, Tamilnadu, 600073, India

    Manikandan Ayyar

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  1. Eva Kaiser
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  2. Saravana Kumar Jaganathan
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  4. Manikandan Ayyar
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Correspondence to Saravana Kumar Jaganathan.

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Kaiser, E., Jaganathan, S.K., Supriyanto, E. et al. Fabrication and characterization of chitosan nanoparticles and collagen-loaded polyurethane nanocomposite membrane coated with heparin for atrial septal defect (ASD) closure. 3 Biotech 7, 174 (2017). https://doi.org/10.1007/s13205-017-0830-6

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  • DOI: https://doi.org/10.1007/s13205-017-0830-6

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