Abstract
A bioselective membrane of the potentiometric sensor based on butyrylcholinesterase and pH-sensitive field-effect transistors (FETs) modified with gold nanoparticles (GNPs) have been developed. In the present investigation, we focused on the study and adjustment of the potentiometric biosensor just for the case of using gold nanoparticles because butyrylcholinesterase has been already used to create the sensors for determination of organophosphorus/carbamate pesticides, heavy metals and glycoalkaloids. The composition of a butyrylcholinesterase-based bioselective membrane with gold nanoparticles was optimized. An influence of the parameters of a work solution on analytical characteristics of biosensor was comprehensively investigated and thoroughly analyzed. An increase of biosensor responses and sensitivity as a result of using GNPs was shown. A highly sensitive, selective biosensor based on immobilized butyrylcholinesterase was developed; its laboratory prototype has linear range of 0.025–0.2 mM, minimum limit of detection of 0.018 mM; response time of 1.4 min. Thus, application of the bioselective membrane modified with gold nanoparticles opens new possibilities to create a new electrochemical biosensors with high sensitivity and stability.
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The work was carried out thanks to financial support from the National Research Foundation of Ukraine in the framework of the competition of projects for research and development "Support of research of leading and young scientists" (project 2020.02/0097).
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Lyudmyla, S., Olena, Z., Valentyna, A. et al. Potentiometric enzyme biosensor modified with gold nanoparticles. Appl Nanosci 13, 5133–5138 (2023). https://doi.org/10.1007/s13204-022-02715-z
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DOI: https://doi.org/10.1007/s13204-022-02715-z