Abstract
Nitrogen nutrition of legume relies on both soil mineral N absorption and symbiotic nitrogen fixation by rhizobia and the symbiotic interaction depends on each of the partners. This study evaluates the variability of symbiotic efficiency on the growth of two cultivars of pea and lentil inoculated with 16 different rhizobial genotypes originating from sub-humid and semi-arid regions of eastern Algeria. The genotypes were defined by the combination of haplotypes characterized by rRNA intergenic spacer (IGS) and a nodulation gene marker (nodD region). Analysis of shoots, roots and nodules is an effective method to examine plants growth. The results revealed that efficiency is linked to the rhizobial genotype, effect of the strains, and host plant species. A statistically significant interaction between cultivar and rhizobial genotype was only found for nodule number, size and biomass in forage pea. With respect tothe development of tested plants, strains having genotypes originating from sub-humid zones are more effective than those originating from semi-arid zones. This functional variability is strongly linked to the genetic variability of the chromosomal ribosomal IGS. Strains isolated from lentil are more effective for lentil growth than those isolated from pea. The nitrogen content correlated with the plant growth. In conclusion, the inoculation of plants cultivated in a semi-arid soils with effective strains from a sub-humid origin is likely to be more beneficial for plants under conditions of environmental stress.
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Acknowledgements
This paper is dedicated to memory of Gisèle Laguerre with which this work was initiated and her guidance in the experimental work. The authors are grateful to Naima Rezkallah, Isabelle Babic and Pierre Tisseyre for technical assistance. We thank Marc Bourso for his help in the glasshouse work. We are very grateful to the director Robin Duponnois of the LSTM, Montpellier, France for having facilitated the realization of this work. This work was supported by the Algerian Ministry of High Education and Scientific Research.
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NR carried out the experiments, analysis of statistical and wrote the manuscript. PL, contributed in the correction and revision of the article and the final approval of the version to be published. KH participated in realising of experiments. All the authors gave intellectual input to the manuscript.
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Riah, N., de Lajudie, P., Béna, G. et al. Variability in symbiotic efficiency with respect to the growth of pea and lentil inoculated with various rhizobial genotypes originating from sub-humid and semi-arid regions of eastern Algeria. Symbiosis 85, 371–384 (2021). https://doi.org/10.1007/s13199-021-00821-0
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DOI: https://doi.org/10.1007/s13199-021-00821-0