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Phylogeny of genera Laminaria and Saccharina (Laminariales, Phaeophyceae) based on three molecular markers

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Abstract

In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae-Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I (COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer (ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina.

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Authors and Affiliations

  1. Ocean University of China, Qingdao, 266003, China

    Shan Chi, Hao Qian, Tianyong Li, Cui Liu, Lei Ren, Xuexi Tang & Tao Liu

  2. Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China

    Xumin Wang

  3. Chinese Academy of Sciences Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100101, China

    Xumin Wang

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  1. Shan Chi
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  2. Hao Qian
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  3. Tianyong Li
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  4. Xumin Wang
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  5. Cui Liu
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  7. Xuexi Tang
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  8. Tao Liu
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Correspondence to Tao Liu.

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Foundation item: The National Natural Science Foundation of China under contract No. 41206116; the Postdoctoral Science Foundation of China under contract No. 2011M501167; the National Public Benefit Research Foundation of China under contract No. 201105021; the Fundamental Research Funds for the Central Universities under contract No. 201362040.

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Chi, S., Qian, H., Li, T. et al. Phylogeny of genera Laminaria and Saccharina (Laminariales, Phaeophyceae) based on three molecular markers. Acta Oceanol. Sin. 33, 139–151 (2014). https://doi.org/10.1007/s13131-014-0525-3

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  • DOI: https://doi.org/10.1007/s13131-014-0525-3

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