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Agaricus bisporus supplementation reduces high-fat diet-induced body weight gain and fatty liver development

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Abstract

Obesity is a global epidemic characterized not only by excessive fat deposition but also by important complications such as nonalcoholic liver steatosis. Beneficial antiobesogenic effects have been described for some mushrooms. The current study aimed to demonstrate the protective effect of Agaricus bisporus (AB) supplementation against the metabolic alterations induced by high-fat-diet (HFD) feeding. Eight-week-old C57BL/6J mice were fed for 10 weeks with one of the following diets: (1) control diet (n = 7), (2) HFD (n = 7), (3) HFD supplemented with 5% AB (n = 9), and (4) HFD supplemented with 10% AB (n = 9). A pair-fed group was also included for the 10% AB group (n = 6). The impact of AB supplementation on food intake, body weight gain, and liver and fat pad weights was examined. Biochemical, histological, and molecular parameters were also analyzed. Dietary supplementation with 10% AB reduced the HFD-induced increase in body, epididymal, and mesenteric fat weights (p < 0.01, p < 0.05, and p < 0.05, respectively). Supplementation with AB also reduced liver damage in a dose-dependent manner (p < 0.01 and p < 0.001). This effect was confirmed by histological analysis that showed that liver steatosis was markedly reduced in mice fed with AB. The beneficial properties of 10% AB supplementation appear to be mediated through a decrease in food intake and via stimulation of mesenteric and hepatic free-fatty acid beta-oxidation, along with a decrease in epidydimal and hepatic expression of CD36. In conclusion, supplementation with AB prevents excessive body weight gain and liver steatosis induced by HFD consumption.

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Acknowledgments

We appreciate the excellent technical assistance of Judith Narro and the helpful advice from Dr. Alfredo Martinez in the histology analysis.

Funding

This work was supported by a grant from the Agencia de Desarrollo Económico de La Rioja (ADER) (project number 2014-I-IDD-00091). ADER played no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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  1. Infectious Diseases, Microbiota and Metabolism Unit, Infectious Diseases Department, Center for Biomedical Research of La Rioja (CIBIR), Piqueras 98, 3rd floor, 26006, Logroño, La Rioja, Spain

    María Iñiguez, Patricia Pérez-Matute, María Jesús Villanueva-Millán, Emma Recio-Fernández & José-Antonio Oteo

  2. Mushroom Technological Research Center of La Rioja (CTICH), Autol, La Rioja, Spain

    Irene Roncero-Ramos & Margarita Pérez-Clavijo

  3. Infectious Diseases Department, Hospital San Pedro, Logroño, La Rioja, Spain

    José-Antonio Oteo

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  1. María Iñiguez
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Correspondence to Patricia Pérez-Matute.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures involving animals were in accordance with the ethical standards of the ethics committee on animal welfare of our institution (Comité Ético de Experimentación Animal del Centro de Investigación Biomédica de La Rioja, CEEA-CIBIR).

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Iñiguez, M., Pérez-Matute, P., Villanueva-Millán, M.J. et al. Agaricus bisporus supplementation reduces high-fat diet-induced body weight gain and fatty liver development. J Physiol Biochem 74, 635–646 (2018). https://doi.org/10.1007/s13105-018-0649-6

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