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Effect of a 2000-m running test on antioxidant and cytokine response in plasma and circulating cells

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Abstract

Exercise intensity usually correlates with increased oxidative stress and enhanced cytokine production. However, it is unknown if all types of exercise that induce muscle damage can cause a parallel response in the oxidation balance and cytokine production. To this end, the effect of a 2000-m running test in a group of volunteers that regularly train in aerobic routines was studied. Different circulating parameters were measured, oxidative stress markers (protein carbonyls and malondialdehyde), antioxidant enzyme activity, and cytokine levels in plasma as well as in the main circulating cells of blood samples obtained in basal conditions and after test execution. As a result, the test caused muscle damage evidenced by an increase in circulating creatine kinase and myoglobin. This was accompanied by an increase in protein carbonyls in plasma and peripheral blood mononuclear cells. Activities of antioxidant enzymes (catalase, glutathione peroxidase and reductase, superoxide dismutase) were elevated in peripheral blood mononuclear cells, neutrophils, and erythrocytes after the test. Regarding cytokine production, interleukin-6, interleukin-8, interleukin-10, and tumor necrosis factor-α exhibited no significant changes after the test. Results suggest that this short but intense running exercise (2000 m) can induce muscle damage and elicit a good balance between oxidant/antioxidant responses with no changes in the circulating concentration of pro-inflammatory cytokines.

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Acknowledgements

We thank Jose Maria Adsuar for his technical assistance in blood analysis. AP, VM, and ER are members of CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/30038) Instituto de Salud Carlos III, Spain. LC-Q and LF were recipients of CONACYT-Mexico (ref 197139) and FPI (Spanish Science Ministry) fellowships respectively. This work was supported by Generalitat Valenciana under the PROMETEO grant 2012/007 to VM and ER.

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Authors and Affiliations

  1. Department of Applied Biology-Nutrition, University Miguel Hernandez, Alicante Institute for Health and Biomedical Research (ISABIAL-FISABIO Foundation), Alicante, Spain

    Lucrecia Carrera-Quintanar & Enrique Roche

  2. Institute for Molecular and Cellular Biology, University Miguel Hernandez, Elche (Alicante), Spain

    Lorena Funes, Miguel Sánchez-Martos & Vicente Micol

  3. Immunology Division, Biotechnology Department, University of Alicante, Alicante, Spain

    Pascual Martinez-Peinado & José M. Sempere

  4. Department for Basic Biology and Health Sciences, University of Balearic Islands, Palma de Mallorca, Spain

    Antoni Pons

  5. CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/30038) Instituto de Salud Carlos III, Madrid, Spain

    Antoni Pons, Vicente Micol & Enrique Roche

  6. Departamento de Biología Aplicada e Instituto de Bioingeniería, Área de Nutrición y Bromatología, Universidad Miguel Hernández, Avda de la Universidad sn., 03202, Elche (Alicante), Spain

    Enrique Roche

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  1. Lucrecia Carrera-Quintanar
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  2. Lorena Funes
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  4. Pascual Martinez-Peinado
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  5. José M. Sempere
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  6. Antoni Pons
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  7. Vicente Micol
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  8. Enrique Roche
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Correspondence to Enrique Roche.

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The protocol was in accordance with local legal requirements and the Helsinki Declaration for research on human beings and approved by the Ethics Committee of the corresponding Autonomic Governments with reference IB 544/05 PI.

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The authors declare that they have no conflict of interest.

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Carrera-Quintanar, L., Funes, L., Sánchez-Martos, M. et al. Effect of a 2000-m running test on antioxidant and cytokine response in plasma and circulating cells. J Physiol Biochem 73, 523–530 (2017). https://doi.org/10.1007/s13105-017-0575-z

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  • DOI: https://doi.org/10.1007/s13105-017-0575-z

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