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Erythropoietin enhances whole body lipid oxidation during prolonged exercise in humans

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Abstract

Animal studies have suggested that erythropoietin, besides its well-known hematopoietic effects, can modulate metabolism and prevent fat accumulation. We investigated the effects of repeated injections of recombinant human erythropoietin (EPO) on the balance of substrate oxidation during aerobic exercise in humans. Twelve healthy aerobically trained males received subcutaneously either moderate dose of EPO (50 U/kg, EPO) or saline injections (NaCl 0.9 %, control) three times a week for 4 weeks. Body weight, % fat, maximal aerobic capacity, and substrate utilization during exercise were assessed before and after treatment, while hemoglobin and hematocrit were monitored regularly during the treatment. Carbohydrate and fat oxidation were evaluated via indirect calorimetry, during a submaximal exercise performed at 75 % of the participants’ maximal aerobic capacity (\( \overset{\cdotp }{V}{O}_{2 \max } \)) for 60 min. Results showed that 4 weeks of EPO treatment significantly enhanced fat oxidation (+56 % in EPO versus −9 % in control) during exercise, independent of its effects on hematological parameters or \( \overset{\cdotp }{V}{O}_{2 \max } \). This study shows that EPO can modulate substrate utilization during exercise, leading to enhanced fat utilization and lower use of carbohydrates. This opens new research directions exploring whether systemic EPO levels, in physiological conditions, participate to the modulation of fat oxidation.

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Acknowledgments

The study has been conducted in the Clinical Physiology Department, Centre Hospitalier Régional Universitaire, Montpellier, France. The authors thank Ray Patton from the University of Sydney for his contribution.

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Authors and Affiliations

  1. Exercise Health and Performance, Faculty of Health Sciences, The University of Sydney, 75 East Street, Lidcombe, NSW, 1825, Sydney, Australia

    Corinne Caillaud

  2. UMR Inserm U1134, Université des Antilles et de la Guyane, Pointe à Pitre, Guadeloupe, France

    Philippe Connes

  3. Laboratory of Excellence GR-Ex “The Red Cell: from genesis to death”, PRES Sorbonne, Paris Cité, France

    Philippe Connes

  4. Institut Universitaire de France, Paris, France

    Philippe Connes

  5. Laboratory of Physiology, Faculty of Medicine of Sousse, University of Sousse, Sousse, Tunisia

    Helmi Ben Saad

  6. Inserm U1046, University of Montpellier 1-CHRU, Montpellier, France

    Jacques Mercier

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  1. Corinne Caillaud
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  2. Philippe Connes
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  3. Helmi Ben Saad
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  4. Jacques Mercier
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Correspondence to Corinne Caillaud.

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Caillaud, C., Connes, P., Ben Saad, H. et al. Erythropoietin enhances whole body lipid oxidation during prolonged exercise in humans. J Physiol Biochem 71, 9–16 (2015). https://doi.org/10.1007/s13105-014-0374-8

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  • DOI: https://doi.org/10.1007/s13105-014-0374-8

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