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Biological Potential of Silver Nanoparticles Synthesized by an Endophytic Fungus Metapochonia suchlasporia-KUMBMDBT-23

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Abstract

The field of fungal nanotechnology has emerged as a novel area of research, leading to significant advancements in the production of nanoparticles with diverse applications for human benefit. This study exploits the endophytic fungus Metapochonia suchlasporia from the medicinal plant Argemone mexicana to biosynthesize Met-AgNPs. M. suchlasporia–submerged fermentation-derived ethyl acetate extract secondary metabolites are also examined. Bio-analytical methods characterized Met-AgNPs. A peak at 417 nm in the UV-visible spectrophotometer absorption spectrum confirmed Met-AgNPs synthesis. FTIR study showed functional group peaks, indicating Met-AgNPs reduction and capping. SEM-EDAX and HR-TEM revealed the nanoparticles’ spherical form, with EDAX revealing silver atoms at 3 keV. XRD confirmed Met-AgNP crystalline structure. DLS and zeta potential measurements showed that synthesized Met-AgNPs were 59.04 nm and stable. Simultaneously, the ethyl acetate extract underwent rigorous analysis to identify secondary metabolites, utilizing UV-visible spectrophotometer, GC-MS, and FTIR techniques. The synthesized Met-AgNPs showed strong antibacterial action against P. aeruginosa (14.06 ± 0.11 mm at 10mg/mL) and excellent antifungal activity against A. brasiliensis (14.1 ± 0.17mm). Also, active Met-AgNPs inhibited DPPH and ABTS with IC50 values of 5.329 and 7.191 μg/mL. Met-AgNPs were cytotoxic up to 52.95% and 46.57% at 200 μg/mL in MTT tests. Nanoparticles also showed significant anti-inflammatory effects in in vitro and in vivo studies. In conclusion, this research strongly supports the pharmaceutical and biomedical potential of the synthesized Met-AgNPs, underscoring their importance as valuable agents in various biomedical applications.

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The authors declare that all relevant data supporting the findings of the study are available within the article.

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Acknowledgments

The authors heartily acknowledge Kuvempu University, Karnataka, INDIA, for financial support to carry out the research work. The Department of Applied Sciences (Nanotechnology), Visvesvaraya Technological University, Bengaluru Region, Muddenahalli, Chikkaballapur, India, for providing facilities to perform FTIR analysis. The Sophisticated Analytical Instrument Facility (SAIF) at Karnatak University Dharwad, Karnataka, India, for providing facilities to perform SEM-EDAX and XRD. The Head Center for Nanotechnology, University Agriculture Sciences (UAS), Raichur, Karnataka, India, for providing facilities to perform particle size analysis by dynamic light scattering (DLS) and Zeta potential. Indian Institute of Science, Bengaluru, Karnataka, India, for providing facilities to perform HR-TEM. Averin Biotech Labs, Bangalore, INDIA., for providing facilities to perform MTT assay.

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This research received no specific grant from any funding agency.

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Authors and Affiliations

  1. Department of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu University, Jnanasahyadri, Shankaraghatta, Shivamogga District, Karnataka, 577451, India

    Manjunatha Dadayya, Sowmya Hirakannavar Veeranna, Nandish Gurubasajar, Akarsh Subhakar & Thippeswamy Basaiah

  2. Department of P.G. Studies and Research in Biochemistry, Kuvempu University, Jnanasahyadri, Shankaraghatta, Shivamogga District, Karnataka, 577451, India

    Megha Gowri Thippeswamy

  3. Department of Studies and Research in Biochemistry, Tumkur University, Tumakuru, Karnataka, 572102, India

    Nagaraju Shivaiah

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  1. Manjunatha Dadayya
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  2. Megha Gowri Thippeswamy
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Contributions

MD—analysis of research work in laboratory, methodology, software, data analysis, and manuscript writing; MGT, NS guided for the experimentation of anti-inflammatory activity and reviewing; SHV—manuscript correction and editing; AS—data interpretation and reviewing; NG—phylogenetic analysis, reviewing, TB—design and correction of research work.

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Correspondence to Thippeswamy Basaiah.

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Approval number was consented to by the Office of Institutional Animal Ethical Committee (IAEC) (Reg. No. 123/PO/C/99/CPCSEA under the rules 5(a) of the “Breeding of experiments on animal (control and supervision rules 1998”, Ref. SSCPT/IAEC.Clear/152/2016-17)).

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Dadayya, M., Gowri Thippeswamy, M., Shivaiah, N. et al. Biological Potential of Silver Nanoparticles Synthesized by an Endophytic Fungus Metapochonia suchlasporia-KUMBMDBT-23. BioNanoSci. 13, 1790–1816 (2023). https://doi.org/10.1007/s12668-023-01177-z

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