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Antibiotic Resistance of Biofilm-Related Catheter-Associated Urinary Tract Isolates of Pseudomonas aeruginosa

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Abstract

Catheter-associated urinary tract infections (CAUTI) are one of the most common nosocomial diseases accounting for up to about 23% of healthcare-associated infections. High antibiotic resistance of biofilm-associated bacteria is a serious limiting factor in the treatment of patients. Therefore, the investigations of bacterial biofilms and their regulation can play a pivotal role in the development of new approaches to treating infections. P. aeruginosa is a key bacterium responsible for most urinary tract infections. In this study, urinary catheter–associated P. aeruginosa strains were isolated and their antibiotic resistance was studied. Polymicrobial biofilms were found on the urinary catheter surfaces derived from patients with different urinary tract diseases. Three strains of Pseudomonas aeruginosa isolated from the catheter-associated biofilms were resistant to meropenem. The resistance to carbapenemss of P. aeruginosa strains 96,347 and 96,349 was observed to be mediated by VIM-type metallo-β-lactamase gene expression.

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Acknowledgements

This paper has been supported by the Kazan Federal University Strategic Academic Leadership Program (PRIORITY-2030). The authors would like to thank the Laboratory of Clinical Bacteriology at the Republican Clinical Hospital of the Republic of Tatarstan for the support in the work with clinical samples.

Funding

This work was funded by the Russian Foundation for Basic Research (research project no.20–315-90093).

Author information

Author notes

  1. Nailya Khabipova and Lia Valeeva equally contributed to this work.

Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan (Volga regoin) University, Kazan, Russia

    Nailya Khabipova, Lia Valeeva, Daniil Kabanov, Viacheslav Vorobev & Margarita Sharipova

  2. Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia

    Elvira Shaidullina

  3. KFU Hospital, the Department of Urology of the Medical and Sanitary Part of Kazan (Volga Region, Federal University, Kazan, Russia

    Zufar Gimadeev

Authors
  1. Nailya Khabipova
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  2. Lia Valeeva
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  4. Daniil Kabanov
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  5. Viacheslav Vorobev
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Contributions

All authors contributed significantly to the current work. L.V. and M.S. designed the experiments. N.K., L.V., E.S., D.K., and V.V. performed the experiments. L.V., N.K., Z.G., and M.S. analyzed data. Z.G. provided the clinical material (catheters). L.V. and N.K. wrote the paper with contributions from all other authors. Correspondence and requests for materials should be addressed to L.V. or M.S. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Lia Valeeva or Margarita Sharipova.

Ethics declarations

Research Involving Humans and Animals Statement

None.

Informed Consent

The materials used in this study do not contain personal data of patients. Informed consent was obtained from all the patients recruited in this study. Clinical samples were provided in accordance with the university ethical regulations.

Conflict of Interest

All authors’ individual rights were respected and information regarding data, pictures and the overall draft was made with their concern.

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Khabipova, N., Valeeva, L., Shaidullina, E. et al. Antibiotic Resistance of Biofilm-Related Catheter-Associated Urinary Tract Isolates of Pseudomonas aeruginosa. BioNanoSci. 13, 1012–1021 (2023). https://doi.org/10.1007/s12668-023-01123-z

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