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Bioinformatic Analysis of a Grimelysin-like Protease in the Klebsiella oxytoca Strain NK-1

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Abstract

Bacterial intracellular proteinases play a relevant role in coordinating various cellular processes. The genome of the uropathogenic Klebsiella oxytoca NK-1 encodes the gene prtKO, homologous to grimelysin of Serratia grimesii. BLAST analysis showed that a lot of bacteria encode grimelysin-like proteinases, known to be mainly distributed in Enterobacterales. Phylogenetic analysis classified these proteins into 10 clusters. PrtKO was predicted to contain conservative motifs typical for grimelysin using bioinformatic tools. In addition, the structure of metalloproteinase-containing genomic loci is conservative among enterobacteria and consists of the genes involved in global physiological processes. Maximal total intracellular proteolytic activity of K. oxytoca NK-1 is observed during the late stationary phase of growth in LB medium at 37 °C. Limited proteolysis of actin indicates the presence of a grimelysin-like proteinase in bacterial cells during the late stationary growth phase. Hence, we present a first report of a new grimelysin-like intracellular metalloproteinase in K. oxytoca.

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Acknowledgements

This work was performed in accordance with the Russian Government Program of Competitive Growth of Kazan Federal University. We express our sincere gratitude to Yaw Akosah for his thoughtful comments and efforts toward improving our manuscript.

Funding

The study was funded by Russian Foundation for Basic Research (Project No. 18–34-00837).

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Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia

    Adeliia G. Giliazeva, Polina S. Misheeva, Margarita R. Sharipova & Ayslu M. Mardanova

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  1. Adeliia G. Giliazeva
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  2. Polina S. Misheeva
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  3. Margarita R. Sharipova
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  4. Ayslu M. Mardanova
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Contributions

A. Mardanova and M. Sharipova conceived and designed the experiments and analyzed the data. Material preparation and data collection were performed by P. Misheeva. A. Giliazeva performed the experiments, analyzed and interpreted the data, and wrote the paper. All authors have read and approved the final manuscript.

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Correspondence to Adeliia G. Giliazeva.

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This article belongs to the Topical Collection: Molecular, Precision and Regenerative Medicine - 2021

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Giliazeva, A.G., Misheeva, P.S., Sharipova, M.R. et al. Bioinformatic Analysis of a Grimelysin-like Protease in the Klebsiella oxytoca Strain NK-1. BioNanoSci. 12, 160–169 (2022). https://doi.org/10.1007/s12668-021-00924-4

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