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Resveratrol-Loaded Microsponge as a Novel Biodegradable Carrier for Dry Powder Inhaler: A New Strategy in Lung Delivery

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Abstract

The aim of the present study was to prepare a resveratrol-loaded microsponge-based dry powder inhaler (RES-MS-DPI) using the quasi-emulsion solvent diffusion method and to explore its suitability for pulmonary delivery. The prepared microsponges were evaluated for physicochemical properties. Additionally, an in vitro lung deposition study was performed using an Andersen cascade impactor (ACI), while in vivo performance was studied by intratracheal administration. The optimized porous and spherical RES-MS showed particle size, surface charge, entrapment efficiency, mass median aerodynamic diameter (MMAD) and fine particle fraction (FPF) of 2.499 ± 0.56 μm, −25.34 mV, 89.45 ± 0.23%, 2.13 ± 0.21 μm and 48.54 ± 0.25%, respectively, with controlled release. Further, in vitro and in vivo studies of RES-MS showed a twofold improvement in the FPF, and 4.4-, 14.12- and 1.94-fold improvement in the area under the curve (AUC), mean residence time (MRT) and Cmax. The RES-MS exhibited higher aerosolization and lung deposition.

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Authors and Affiliations

  1. Department of Pharmaceutics, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune, Maharashtra, 411038, India

    Ashwin J. Mali, Anamika Rokade, Ravindra Kamble, Atmaram Pawar & C. Bothiraja

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  1. Ashwin J. Mali
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  2. Anamika Rokade
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  3. Ravindra Kamble
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  4. Atmaram Pawar
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  5. C. Bothiraja
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Correspondence to Ashwin J. Mali.

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Research Involving Humans and Animals Statement

This research proposal was prepared according to the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). The experimental protocol was approved by the Institutional Animal Ethics Committee (IAEC) of Poona College of Pharmacy, Pune, India (CPCSEA/PCP/PCT25/2018-19).

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Mali, A.J., Rokade, A., Kamble, R. et al. Resveratrol-Loaded Microsponge as a Novel Biodegradable Carrier for Dry Powder Inhaler: A New Strategy in Lung Delivery. BioNanoSci. 11, 32–43 (2021). https://doi.org/10.1007/s12668-020-00800-7

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