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Genetic polymorphisms in phase II metabolizing enzymes in alcoholic and idiopathic chronic pancreatitis: Indian scenario

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Indian Journal of Gastroenterology Aims and scope Submit manuscript

Abstract

Aim

To study polymorphisms in glutathione-S-transferases (GST-T1, GST-M1, GST-P1) and uridine-5′-diphosphate-glucuronosyl-transferases (UGT1A7) genes and the risk of developing chronic pancreatitis (CP) associated with these polymorphisms.

Methods

This study included 49 alcoholic and 51 idiopathic chronic pancreatitis patients, 50 alcohol addicts and 50 healthy controls. Polymorphism(s) in GST-T1 and GST-M1 genes were assessed by multiplex polymerase chain reaction (PCR), while PCR–radiofrequency lesioning (RFLP) was employed to assess the same in GST-P1 and UGT1A7 genes. The differences in polymorphism frequency between groups and the risk of developing pancreatitis were assessed by the odds ratio.

Results

Strong association of the null genotype of GST-T1 with CP susceptibility was observed. Alcoholics with the Val allele of GST-P1 have higher chances of having pancreatitis. Idiopathic pancreatitis patients with higher age at the onset of pain were found to have the null genotype of GST-M1.

Conclusion

Alcoholics with the null genotype of the GST-T1 gene and the Valine allele of the GST-P1 gene are at a higher risk of developing CP. Thus, genotyping of these genes may serve as an important screening tool for the identification of high-risk groups among alcoholics.

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Funding

The study has been funded as an Intramural Grant by the Research Section, All India Institute of Medical Sciences, New Delhi, India (F.8–115/A-115/2011/RS).

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Authors and Affiliations

  1. Department of Gastroenterology and Human Nutrition Unit, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Nidhi Singh, Safoora Rashid, Sumaira Rashid & Anoop Saraya

  2. Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Hem Chandra Sati

  3. Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Surabhi Gupta

  4. National Drug Dependence Treatment Centre, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Meera Vaswani

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  1. Nidhi Singh
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Correspondence to Anoop Saraya.

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NS, SR, HCS, SG, MV and AS declare no competing interests.   

Ethics statement

The study was performed conforming to the Helsinki Declaration of 1975, as revised in 2000 and 2008 concerning human and animal rights, and the authors followed the policy concerning informed consent as shown on Springer.com.

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Singh, N., Rashid, S., Rashid, S. et al. Genetic polymorphisms in phase II metabolizing enzymes in alcoholic and idiopathic chronic pancreatitis: Indian scenario. Indian J Gastroenterol 42, 199–208 (2023). https://doi.org/10.1007/s12664-022-01320-w

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  • DOI: https://doi.org/10.1007/s12664-022-01320-w

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