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Association of Helicobacter pylori with hepatobiliary stone disease, a prospective case control study

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Abstract

Background

Hepatobiliary stone disease is one of the most common surgical conditions worldwide. There are multiple causative agents responsible for the formation of hepatobiliary stones, and bacterial infection is one of them. The presence of Helicobacter DNA species has been investigated in the biliary epithelium of patients with biliary diseases. However, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confounding factors, or ethical and regional differences among populations.

Methods

We investigated the presence of Helicobacter pylori species by PCR of 26-kDa surface antigen specific to H. pylori in bile samples from 50 cases with hepatobiliary stones and 25 controls without hepatobiliary stones. The control group comprised of 20 patients of hydatid cyst disease of liver and 5 patients of right colonic growth.

Result

H. pylori was present in 20 bile samples among cases and was absent in 30 bile samples among cases. Among controls, H. pylori could not be detected. A significant association of the presence of H. pylori with hepatobiliary stone disease was seen (p < 0.001).

Conclusion

There is an association between bile infection with H. pylori and hepatobiliary stone disease.

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References

  1. Kawaguchi M, Saito T, Ohno H, et al. Bacteria closely resembling Helicobacter pylori detected immune histologically and genetically in resected gallbladder mucosa. J Gastroenterol. 1996;31:294–8.

    Article  CAS  PubMed  Google Scholar 

  2. Monti J, Fay M, Banchio C, et al. Detection of Helicobacter pylori by polymerase reaction in bile samples from gallbladder and bile stones. Acta Gastroenterol Latinoam. 1999;29:251–3.

    CAS  PubMed  Google Scholar 

  3. Farshad S, Alborzi A, Malek Hosseini SA, et al. Identification of Helicobacter pylori DNA in Iranian patients with gallstones. Epidemiol Infect. 2004;132:1185–9.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Abayli B, Colakoglu S, Serin M, et al. Helicobacter pylori in the etiology of cholesterol gallstones. J Clin Gastroenterol. 2005;39:134–7.

    CAS  PubMed  Google Scholar 

  5. Lin TT, Yeh CT, Wu CS, Liaw YF. Detection and partial sequence analysis of Helicobacter pylori DNA in the bile samples. Dig Dis Sci. 1995;40:2214–9.

    Article  CAS  PubMed  Google Scholar 

  6. Abd-Al Kareem AY. Gallstones associated with bacterial incidence. Tikrit J Pure Sci. 2011;16(4).

  7. Csendes A, Becerra M, Burdiles P, Demian I, Bancalari K, Csendes P. Bacteriological studies of bile from the gallbladder in patients with carcinoma of the gall bladder, cholelithiasis, common bile duct stones and no gallstones disease. Eur J Surg. 1994;160:363–7.

    CAS  PubMed  Google Scholar 

  8. Bickley J, Owen RJ, Fraser AG, Pounder RE. Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque. J Med Microbiol. 1993;39:338–44.

    Article  CAS  PubMed  Google Scholar 

  9. Hammar M, Tyszkiewicz T, Wadström T, O'Toole PW. Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction. J Clin Microbiol. 1992;30:54–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Silva CP, Pereira-Lima JC, Oliveira AG, et al. Association of the presence of Helicobacter in gallbladder tissue with cholelithiasis and cholecystitis. J Clin Microbiol. 2003;41:5615–8.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Mendez-Sanchez N, Pichardo R, Gonzalez J, et al. Lack of association between Helicobacter sp colonization and gallstone disease. J Clin Gastroenterol. 2001;32:138–41.

    Article  CAS  PubMed  Google Scholar 

  12. Roosendaal R, Kuipers EJ, Vandenbroucke-Grauls CM, Kusters JG. Helicobacter species are not detectable by 16S rDNA PCR in bile from Dutch patients with common bile duct stones. Digestion. 2002;66:89–91.

  13. Fox JG, Dewhirst FE, Shen Z, et al. Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology. 1998;114:755–63.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of General and Minimal Invasive Surgery, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190 010, India

    Mohammad Yousuf Dar & Mubashir A Shah

  2. Department of Surgical Gastroenterology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190 010, India

    Sadaf Ali & O J Shah

  3. Department of Internal Medicine, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190 010, India

    Abdul Hameed Raina

  4. Department of Clinical Biochemistry, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190 010, India

    Manzoor A Raina & Syed Mudassar

Authors
  1. Mohammad Yousuf Dar
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  2. Sadaf Ali
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  3. Abdul Hameed Raina
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  4. Manzoor A Raina
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  5. O J Shah
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  6. Mubashir A Shah
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  7. Syed Mudassar
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Corresponding author

Correspondence to Mohammad Yousuf Dar.

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Conflict of interest

MYD, SA, AHR, MAR, OJS, MAS, and SM declare that they have no conflict of interest.

Ethics statement

The study was performed in a manner to confirm with the Helsinki Declaration of 1975, as revised in 2000 and 2008 concerning human and animal rights. Also, the authors followed the policy concerning informed consent as shown on the Springer publishing group.

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Dar, M.Y., Ali, S., Raina, A.H. et al. Association of Helicobacter pylori with hepatobiliary stone disease, a prospective case control study. Indian J Gastroenterol 35, 343–346 (2016). https://doi.org/10.1007/s12664-016-0675-7

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  • DOI: https://doi.org/10.1007/s12664-016-0675-7

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